Jakovljević Danijel, Nikolić Milan, Jovanović Vesna, Vidonja Uzelac Teodora, Nikolić-Kokić Aleksandra, Novaković Emilija, Miljević Čedo, Milovanović Maja, Blagojević Duško
Department of Biochemistry, Faculty of Chemistry, University of Belgrade, 11158 Belgrade, Serbia.
Department of Physiology, Institute for Biological Research "Siniša Stanković", National Institute of Republic of Serbia, University of Belgrade, 11108 Belgrade, Serbia.
Pharmaceuticals (Basel). 2024 Jan 18;17(1):130. doi: 10.3390/ph17010130.
Epilepsy is a chronic brain disease affecting millions of people worldwide, but little is known about the impact of anti-seizure medications on redox homeostasis.
This study aimed to compare the effects of the long-term use of oral anti-seizure medications in monotherapy (lamotrigine, carbamazepine, and valproate) on antioxidant enzymes: superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, haemoglobin, and methaemoglobin content in erythrocytes, and concentrations of total proteins and thiols, nitrites, lipid peroxides and total glutathione in the plasma of epilepsy patients and drug-naïve patients.
The results showed that lamotrigine therapy led to lower superoxide dismutase activity ( < 0.005) and lower concentrations of total thiols ( < 0.01) and lipid peroxides ( < 0.01) compared to controls. On the other hand, therapy with carbamazepine increased nitrite levels ( < 0.01) but reduced superoxide dismutase activity ( < 0.005). In the valproate group, only a decrease in catalase activity was observed ( < 0.005). Canonical discriminant analysis showed that the composition of antioxidant enzymes in erythrocytes was different for both the lamotrigine and carbamazepine groups, while the controls were separated from all others.
Monotherapy with anti-seizure medications discretely alters redox homeostasis, followed by distinct relationships between antioxidant components.
癫痫是一种慢性脑部疾病，影响着全球数百万人，但关于抗癫痫药物对氧化还原稳态的影响却知之甚少。
本研究旨在比较长期单一使用口服抗癫痫药物（拉莫三嗪、卡马西平和丙戊酸盐）对癫痫患者和未服用过药物患者红细胞中的抗氧化酶（超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、血红蛋白和高铁血红蛋白含量）以及血浆中总蛋白、硫醇、亚硝酸盐、脂质过氧化物和总谷胱甘肽浓度的影响。
结果显示，与对照组相比，拉莫三嗪治疗导致超氧化物歧化酶活性降低（<0.005），总硫醇浓度降低（<0.01），脂质过氧化物浓度降低（<0.01）。另一方面，卡马西平治疗可提高亚硝酸盐水平（<0.01），但会降低超氧化物歧化酶活性（<0.005）。在丙戊酸盐组中，仅观察到过氧化氢酶活性降低（<0.005）。典型判别分析表明，拉莫三嗪组和卡马西平组红细胞中抗氧化酶的组成不同，而对照组与其他所有组均有区别。
抗癫痫药物单一疗法会离散地改变氧化还原稳态，随后抗氧化成分之间会呈现出不同的关系。
