Tanaka M, Shigetani Y, Sugiyama S, Tamura K, Nakamura H, Ide H
Biological Institute, Tohoku University, Aoba, Sendai, Japan.
Dev Growth Differ. 1998 Aug;40(4):423-9. doi: 10.1046/j.1440-169x.1998.t01-2-00007.x.
In the early chick embryo, the dorsal ventral (DV) boundary organizes the apical ectodermal ridge (AER) structure in the limb bud field. Here it is reported that Engrailed-1 (En-1), a homolog of the Drosophila segment polarity gene engrailed expressed in the ventral limb ectoderm, participates in AER formation at the DV boundary of the limb bud. Restricted ectopic expression of En-1 in the dorsal side of the limb bud by transplantation of En-1-overexpressing ectoderm induces ectopic AER at the boundary of En-1-positive and -negative cells. The results suggest that En-1 is involved in AER formation at the DV boundary of the limb bud.
在早期鸡胚中，背腹（DV）边界在肢芽区域组织顶端外胚层嵴（AER）结构。本文报道，在腹侧肢外胚层表达的果蝇节段极性基因engrailed的同源物Engrailed-1（En-1）参与肢芽DV边界处AER的形成。通过移植过表达En-1的外胚层，使En-1在肢芽背侧进行局限性异位表达，可在En-1阳性和阴性细胞的边界诱导异位AER。结果表明，En-1参与肢芽DV边界处AER的形成。
