Jain S K, Krueger K S, McVie R, Jaramillo J J, Palmer M, Smith T
Department of Pediatrics, Louisiana State University Medical Center, Shreveport 71130, USA.
Diabetes Care. 1998 Sep;21(9):1511-6. doi: 10.2337/diacare.21.9.1511.
To study the effect of vitamin E supplementation on platelet hyperaggregability in type 1 diabetic patients.
Written informed consent according to the Institutional Review Board on Human Experimentation guidelines was obtained from diabetic patients (n = 29) and their age-matched normal siblings (n = 21) to participate in this study. Diabetic patients were supplemented with DL-alpha-tocopherol (vitamin E) capsule (orally, 100 IU/day) or placebo for 3 months in a double-blind clinical trial. Alternate diabetic patients were assigned to vitamin E or placebo during regular visits to the clinic. Fasting blood was collected from each diabetic patient before the start and after the vitamin E or placebo supplementation. Platelet aggregability was assessed by competitive enzyme-linked immunosorbent assay of the blood TxB2 (a stable thromboxane metabolite). Plasma vitamin E and MDA (malondialdehyde, a product of lipid peroxidation) was assessed by high-performance liquid chromatography. Data were analyzed statistically on 12 diabetic patients on vitamin E and 12 on placebo supplementation.
Diabetic patients (n = 29) had 62% higher (P < 0.05) levels of TxB2 and 15% higher levels (P < 0.05) of MDA in comparison to normal subjects (n = 21). Plasma TxB2 levels had a significant correlation with MDA levels (r = 0.45, P < 0.02) but not with the HbA1 (r = -0.08), glucose (r = -0.13), duration of diabetes (r = -0.04), or age (r = 0.12) of diabetic patients. Vitamin E supplementation lowered MDA levels by 30% (P < 0.04), TxB2 levels by 51% (P < 0.03), and triglyceride levels by 22% (P < 0.04) in diabetic patients. There were no differences in these parameters before versus after placebo supplementation.
The elevated blood level of TxB2 (hyperaggregability of platelets) is significantly related to the level of lipid peroxidation products (oxidative stress) in type 1 diabetic patients. Supplementation of modest doses of vitamin E (100 IU/day) significantly lowers blood TxB2 and lipid peroxidation products levels in type 1 diabetic patients.
研究补充维生素E对1型糖尿病患者血小板高聚集性的影响。
根据人体实验机构审查委员会的指导方针，获得了糖尿病患者（n = 29）及其年龄匹配的正常同胞（n = 21）参与本研究的书面知情同意书。在一项双盲临床试验中，糖尿病患者口服DL-α-生育酚（维生素E）胶囊（100 IU/天）或安慰剂，为期3个月。在定期门诊时，交替将糖尿病患者分配至维生素E组或安慰剂组。在补充维生素E或安慰剂之前及之后，采集每位糖尿病患者的空腹血样。通过竞争性酶联免疫吸附测定法检测血液中的TxB2（一种稳定的血栓素代谢产物）来评估血小板聚集性。通过高效液相色谱法评估血浆维生素E和丙二醛（脂质过氧化产物）。对12例补充维生素E的糖尿病患者和12例补充安慰剂的糖尿病患者的数据进行统计学分析。
与正常受试者（n = 21）相比，糖尿病患者（n = 29）的TxB2水平高62%（P < 0.05），丙二醛水平高15%（P < 0.05）。血浆TxB2水平与丙二醛水平显著相关（r = 0.45，P < 0.02），但与糖尿病患者的糖化血红蛋白（r = -0.08）、血糖（r = -0.13）、糖尿病病程（r = -0.04）或年龄（r = 0.12）无关。补充维生素E可使糖尿病患者的丙二醛水平降低30%（P < 0.04），TxB2水平降低51%（P < 0.03），甘油三酯水平降低22%（P < 0.04）。补充安慰剂前后这些参数无差异。
1型糖尿病患者血液中TxB2水平升高（血小板高聚集性）与脂质过氧化产物水平（氧化应激）显著相关。补充适量剂量的维生素E（100 IU/天）可显著降低1型糖尿病患者血液中的TxB2和脂质过氧化产物水平。
