Kuczius T, Haist I, Groschup M H
Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
J Infect Dis. 1998 Sep;178(3):693-9. doi: 10.1086/515337.
Five mouse scrapie strains, a mouse-passaged scrapie isolate derived from a field case in sheep in Germany, and 2 mouse-passaged bovine spongiform encephalopathy (BSE) isolates were analyzed by immunoblot in regards to banding patterns of proteinase K-digested pathologic prion proteins (PrPres). To obtain reliable results, the photo-imager technique was used for measurement of staining band intensities. Distinct and reproducible profiles were observed for the different strains or isolates. A British and a German BSE isolate were similar, suggesting the same source of infection. The German scrapie isolate resembled scrapie strain ME7, which has frequently been isolated from sheep scrapie in the past. In selected strains or isolates, no influence of the mouse lines used was observed on PrPres profiles, nor were brain region-specific differences apparent. This investigation suggests that PrPres glycotyping can be an invaluable tool for the in vitro differentiation of BSE and scrapie isolates.
对5种小鼠瘙痒病毒株、1种源自德国绵羊田间病例的经小鼠传代的瘙痒病分离株以及2种经小鼠传代的牛海绵状脑病（BSE）分离株，就蛋白酶K消化后的病理性朊病毒蛋白（PrPres）的条带模式进行了免疫印迹分析。为获得可靠结果，采用了光成像技术来测量染色条带强度。观察到不同毒株或分离株具有独特且可重复的图谱。一种英国BSE分离株和一种德国BSE分离株相似，表明感染源相同。德国瘙痒病分离株与瘙痒病毒株ME7相似，ME7过去经常从绵羊瘙痒病中分离得到。在选定的毒株或分离株中，未观察到所用小鼠品系对PrPres图谱有影响，脑区特异性差异也不明显。这项研究表明，PrPres糖基分型可能是体外区分BSE和瘙痒病分离株的一项非常有价值的工具。
