Bousso P, Casrouge A, Altman J D, Haury M, Kanellopoulos J, Abastado J P, Kourilsky P
Unité de Biologie Moléculaire du Géne INSERM U277, Institut Pasteur, Paris, France.
Immunity. 1998 Aug;9(2):169-78. doi: 10.1016/s1074-7613(00)80599-3.
Previous studies have analyzed the diversity of T cell responses upon immunization. Little is known, however, about the individual variability of naive repertoires and its influence on immune responses. In the present study, T cells specific for a Kd-restricted epitope derived from HLA-A2 were purified from individual immunized mice using tetramers of MHC-peptide. Their TCRbeta chains were sequenced revealing strong biases but large variations in BJ usage and clonal composition. Most importantly, sequence analysis from nonimmunized mice demonstrated the preexistence of a small set of splenic precursors, distinct in each mouse and comprising less than 200 cells. Therefore, differences in precursor pools appear to be the major source of individual variability in antigen-selected repertoires.
以往的研究分析了免疫后T细胞反应的多样性。然而，关于初始库的个体变异性及其对免疫反应的影响却知之甚少。在本研究中，使用MHC-肽四聚体从个体免疫小鼠中纯化出对源自HLA-A2的Kd限制性表位具有特异性的T细胞。对其TCRβ链进行测序，结果显示在BJ使用和克隆组成方面存在强烈的偏向性但也有很大差异。最重要的是，对未免疫小鼠的序列分析表明，存在一小部分脾脏前体细胞，每只小鼠中的这些细胞都不同，且数量少于200个。因此，前体库的差异似乎是抗原选择库中个体变异性的主要来源。
