Bomsel M, Heyman M, Hocini H, Lagaye S, Belec L, Dupont C, Desgranges C
U332 INSERM ICGM, Paris, France.
Immunity. 1998 Aug;9(2):277-87. doi: 10.1016/s1074-7613(00)80610-x.
Human immunodeficiency virus, generated during contact between HIV-infected cells and the apical surface of an epithelial cell, can cross a tight epithelial barrier by transcytosis. We show that transcytosis of primary HIV isolates is blocked by dimeric IgA or IgM against HIV envelope proteins. Neutralization occurs intracellularly within the apical recycling endosome, and immune complexes are specifically recycled to the mucosal surface. One epitope involved in neutralization is a conserved sequence of the gp41 HIV envelope protein subunit. Finally, transcytosis also occurs across functional human mucosal tissue in a process inhibited by a serosal internalization of IgM against the HIV envelope protein. These results suggest that induction of mucosal immunity to HIV envelope proteins may impair the transcytotic route of HIV mucosal transmission.
在HIV感染细胞与上皮细胞顶端表面接触过程中产生的人类免疫缺陷病毒，可通过转胞吞作用穿过紧密的上皮屏障。我们发现，针对HIV包膜蛋白的二聚体IgA或IgM可阻断原代HIV分离株的转胞吞作用。中和作用发生在顶端循环内体的细胞内，免疫复合物被特异性地循环至黏膜表面。参与中和作用的一个表位是gp41 HIV包膜蛋白亚基的保守序列。最后，转胞吞作用也会在功能性人类黏膜组织中发生，这一过程会受到针对HIV包膜蛋白的IgM浆膜内化作用的抑制。这些结果表明，诱导针对HIV包膜蛋白的黏膜免疫可能会损害HIV黏膜传播的转胞吞途径。
