β-半乳糖苷酶的氨基酸序列。V. 胰凝乳蛋白酶消化肽段的分离与序列分析
Amino acid sequence of beta-galactosidase. V. Isolation and sequences of chymotryptic peptides.
作者信息
Fowler A V
出版信息
J Biol Chem. 1978 Aug 10;253(15):5484-9.
The amino acid sequence of 72 chymotryptic peptides isolated from 14C-, 3H-labeled carboxymethyl-beta-galactosidase has been determined. A variety of techniques were used in the isolation procedures including separation by solubility, size, and ion exchange and paper chromatography. These peptides contain approximatley 500 amino acids, range in size from 2 to 26 residues, and give overlaps with tryptic peptides of 16 to 55 residues. Peptides from this digest and those reported earlier from tryptic digests account together for the sequence of about 600 of the 1021 residues in the subunit.
已确定从经¹⁴C、³H标记的羧甲基-β-半乳糖苷酶中分离出的72个胰凝乳蛋白酶肽段的氨基酸序列。在分离过程中使用了多种技术，包括通过溶解度、大小、离子交换和纸色谱法进行分离。这些肽段包含约500个氨基酸，大小从2到26个残基不等，并且与16到55个残基的胰蛋白酶肽段有重叠。来自该消化物的肽段和先前报道的来自胰蛋白酶消化物的肽段共同构成了亚基中1021个残基中约600个残基的序列。
Zotero 插件