Yang B C, Liu C W, Chen Y C, Yu C K
Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China.
Immunol Invest. 1998 Jul-Sep;27(4-5):291-302. doi: 10.3109/08820139809070902.
The onset of lupus-like disease in NZB/NZW F1 mice was correlated with the expression of IL-10 at 4 m of age, and with a sequential enhanced expression of IFN-gamma and IL-6 between 6 to 8 m of age. The expression of IFN-gamma and IL-6 was associated with exacerbation of disease symptom, production of anti-DNA antibody, and increase in total serum IgG1. Exogenous dehydroepiandrosterone (DHEA) given in animal diet significantly prolonged survival, and delayed formation of autoantibody of NZB/NZW F1 mice as compared to mice fed on control diet. The effect of DHEA paralleled a delay in the expression of IL-10 and IL-6 and an earlier detection of IL-12 transcripts. Moreover, DHEA-fed mice had higher serum IgG2a level than control diet-fed mice. Collectively, DHEA may modify the activation of distinct subset of T helper cells in NZB/NZW F1 mice at different phases of disease progression.
NZB/NZW F1小鼠狼疮样疾病的发病与4月龄时IL-10的表达相关，且与6至8月龄期间IFN-γ和IL-6的表达依次增强有关。IFN-γ和IL-6的表达与疾病症状加重、抗DNA抗体产生以及血清总IgG1增加相关。与喂食对照饮食的小鼠相比，在动物饮食中添加外源性脱氢表雄酮（DHEA）可显著延长NZB/NZW F1小鼠的生存期，并延迟其自身抗体的形成。DHEA的作用与IL-10和IL-6表达的延迟以及IL-12转录本的更早检测平行。此外，喂食DHEA的小鼠血清IgG2a水平高于喂食对照饮食的小鼠。总体而言，DHEA可能在疾病进展的不同阶段改变NZB/NZW F1小鼠中不同亚群辅助性T细胞的激活。
