Yoon T J, Yoo Y C, Kang T B, Baek Y J, Huh C S, Song S K, Lee K H, Azuma I, Kim J B
Animal Resources Research Center, College of Animal Husbandry, Kon-Kuk University, Seoul, Korea.
Int J Immunopharmacol. 1998 Apr-May;20(4-5):163-72. doi: 10.1016/s0192-0561(98)00024-1.
We here demonstrated the prophylactic effect of an extract (KM-110) from Viscum album coloratum, a Korean mistletoe, on tumor metastasis produced by highly metastatic tumor cells, colon 26-M3.1 carcinoma, B16-BL6 melanoma and L5178Y-ML25 lymphoma cells, using experimental models in mice. Intravenous (i.v.) administration of KM-110 (100 microg/mouse) 2 days before tumor inoculation significantly inhibited lung metastasis of B16-BL6 and colon 26-M3.1 cells, and liver and spleen metastasis of L5178Y-ML25 cells. The prophylactic effect of KM-110 on tumor metastasis was evident with various administration routes, i.e. subcutaneous, oral, intranasal as well as i.v., and was dependent upon the dose of KM-110 administered. Furthermore, mice given KM-110 (100 microg) 2 days before tumor inoculation showed significantly prolonged survival rates compared with the untreated mice. In a time course analysis of NK activity, i.v. administration of KM-110 (100 microg) significantly augmented NK cytotoxicity to Yac-a tumor cells from 1 to 3 days after KM-110 treatment. Furthermore, depletion NK cells by injection of rabbit anti-asialo GM1 serum completely abolished the inhibitory effect of KM-110 on lung metastasis of colon 26-M3.1 cells. These results suggest that KM-110 possesses immunopotentiating activity which enhances the host defense system against tumors, and that its prophylactic effect on tumor metastasis is mediated by NK cell activation.
我们在此利用小鼠实验模型，证明了韩国槲寄生——白果槲寄生提取物（KM-110）对高转移性肿瘤细胞（结肠26-M3.1癌、B16-BL6黑色素瘤和L5178Y-ML25淋巴瘤细胞）产生的肿瘤转移具有预防作用。在肿瘤接种前两天静脉注射（i.v.）KM-110（100微克/小鼠）可显著抑制B16-BL6和结肠26-M3.1细胞的肺转移，以及L5178Y-ML25细胞的肝和脾转移。KM-110对肿瘤转移的预防作用在多种给药途径下均很明显，即皮下、口服、鼻内以及静脉注射，且取决于所给予的KM-110剂量。此外，在肿瘤接种前两天给予KM-110（100微克）的小鼠与未治疗的小鼠相比，存活率显著延长。在对自然杀伤（NK）活性的时间进程分析中，静脉注射KM-110（100微克）在KM-110治疗后1至3天显著增强了对Yac-a肿瘤细胞的NK细胞毒性。此外，通过注射兔抗去唾液酸GM1血清耗尽NK细胞完全消除了KM-110对结肠26-M3.1细胞肺转移的抑制作用。这些结果表明，KM-110具有免疫增强活性，可增强宿主对肿瘤的防御系统，并且其对肿瘤转移的预防作用是由NK细胞激活介导的。
