Yoon Taek Joon, Yoo Yung Choon, Kang Tae Bong, Song Seong Kyu, Lee Kyung Bok, Her Erk, Song Kyung Sik, Kim Jong Bae
Institute for Biomedical Research, Han Dong University, Namsong-Ri 3, Buk-Ku, Pohang, Korea.
Arch Pharm Res. 2003 Oct;26(10):861-7. doi: 10.1007/BF02980033.
Inhibitory effect of the lectins (KML-C) isolated from Korean mistletoe (KM; Viscum album coloratum) on tumor metastases produced by murine tumor cells (B16-BL6 melanoma, colon 26-M3.1 carcinoma and L5178Y-ML25 lymphoma cells) was investigated in syngeneic mice. An intravenous (i.v.) administration of KML-C (20-50 ng/mouse) 2 days before tumor inoculation significantly inhibited lung metastases of both B16-BL6 and colon 26-M3.1 cells. The prophylactic effect of 50 ng/mouse of KML-C on lung metastasis was almost the same with that of 100 microg/mouse of KM. Treatment with KML-C 1 day after tumor inoculation induced a significant inhibition of not only the experimental lung metastasis induced by B16-BL6 and colon 26-M3.1 cells but also the liver and spleen metastasis of L5178Y-ML25 cells. Furthermore, multiple administration of KML-C given at 3 day-intervals after tumor inoculation led to a significant reduction of lung metastasis and suppression of the growth of B16-BL6 melanoma cells in a spontaneous metastasis model. In an assay for natural killer (NK) cell activity, i.v. administration of KML-C (50 ng/mouse) significantly augmented NK cytotoxicity against Yac-1 tumor cells 2 days after KML-C treatment. In addition, treatment with KML-C (50 ng/mouse) induced tumoricidal activity of peritoneal macrophages against B16-BL6 and 3LL cells. These results suggest that KML-C has an immunomodulating activity to enhance the host defense system against tumors, and that its prophylactic and therapeutic effect on tumor metastasis is associated with the activation of NK cells and macrophages.
研究了从韩国槲寄生（KM；白果槲寄生）中分离出的凝集素（KML-C）对同基因小鼠体内鼠肿瘤细胞（B16-BL6黑色素瘤、结肠26-M3.1癌和L5178Y-ML25淋巴瘤细胞）产生的肿瘤转移的抑制作用。在肿瘤接种前2天静脉注射（i.v.）KML-C（20-50 ng/小鼠）可显著抑制B16-BL6和结肠26-M3.1细胞的肺转移。50 ng/小鼠的KML-C对肺转移的预防效果与100 μg/小鼠的KM几乎相同。在肿瘤接种后1天用KML-C治疗不仅显著抑制了B16-BL6和结肠26-M3.1细胞诱导的实验性肺转移，还抑制了L5178Y-ML25细胞的肝转移和脾转移。此外，在肿瘤接种后以3天为间隔多次给予KML-C可导致自发转移模型中肺转移的显著减少和B16-BL6黑色素瘤细胞生长的抑制。在自然杀伤（NK）细胞活性测定中，静脉注射KML-C（50 ng/小鼠）在KML-C治疗2天后显著增强了对Yac-1肿瘤细胞的NK细胞毒性。此外,用KML-C（50 ng/小鼠）治疗可诱导腹膜巨噬细胞对B16-BL6和3LL细胞的杀瘤活性。这些结果表明，KML-C具有免疫调节活性，可增强宿主对肿瘤的防御系统，其对肿瘤转移的预防和治疗作用与NK细胞和巨噬细胞的激活有关。
