Marttila H J, Soini H, Vyshnevskiy B I, Otten T F, Vasilyef A V, Huovinen P, Viljanen M K
Mycobacterial Reference Laboratory, Turku Immunology Centre, National Public Health Institute, Finland.
Scand J Infect Dis. 1998;30(2):129-32. doi: 10.1080/003655498750003492.
Resistance to rifampin (RMP) is associated with mutations in the rpoB gene. Disk elution method, direct DNA sequencing and line probe assay were compared in rapid detection of rpoB mutations from 30 Mycobacterium tuberculosis isolates. Concordance between LiPA and culture was 93.3%, whereas sequencing yielded 100% concordance with culture. These results indicate that line probe assay is nearly as sensitive a method as sequencing in rapid detection of RMP-resistance of M. tuberculosis isolates, and can be applied in laboratories working with standard PCR equipment.
对利福平（RMP）的耐药性与rpoB基因突变有关。对30株结核分枝杆菌分离株的rpoB基因突变进行快速检测时，比较了纸片洗脱法、直接DNA测序法和线性探针分析法。线性探针分析法（LiPA）与培养结果的一致性为93.3%，而测序法与培养结果的一致性为100%。这些结果表明，在快速检测结核分枝杆菌分离株的利福平耐药性方面，线性探针分析法几乎与测序法一样灵敏，并且可应用于配备标准PCR设备的实验室。
