Mäkinen Johanna, Marttila Harri J, Marjamäki Merja, Viljanen Matti K, Soini Hanna
Mycobacterial Reference Laboratory, National Public Health Institute, Kiinamyllynkatu 13, FI-20520 Turku, Finland.
J Clin Microbiol. 2006 Feb;44(2):350-2. doi: 10.1128/JCM.44.2.350-352.2006.
Two commercially available DNA line probe assays, Genotype MTBDR and INNO-LiPA Rif. TB, were evaluated for their abilities to detect resistance to isoniazid (INH) and rifampin (RIF) in 52 Mycobacterium tuberculosis isolates. The test results were compared to those obtained by phenotypic drug susceptibility testing and sequencing. Compared to the results of phenotypic drug susceptibility testing, the Genotype MTBDR test results were concordant for INH for 47 of the 52 (90.4%) isolates, and both the Genotype MTBDR and the INNO-LiPA Rif. TB test results were concordant for RIF for 51 of the 52 (98.1%) isolates. The Genotype MTBDR test results correlated with the sequencing results for 48 of the 52 (92.3%) isolates and the INNO-LiPA Rif. TB results for 50 of the 52 (96.2%) isolates. Both assays are useful for the rapid screening of M. tuberculosis isolates obtained from patients suspected of having multidrug-resistant tuberculosis, but the GenoType MTBDR assay has the advantage of being able to detect resistance to both INH and RIF simultaneously.
对两种市售的DNA线性探针检测方法(即Genotype MTBDR和INNO-LiPA Rif.TB)检测52株结核分枝杆菌分离株对异烟肼(INH)和利福平(RIF)耐药性的能力进行了评估。将检测结果与通过表型药物敏感性试验和测序获得的结果进行比较。与表型药物敏感性试验结果相比,Genotype MTBDR检测结果在52株分离株中的47株(90.4%)对INH检测结果一致,Genotype MTBDR和INNO-LiPA Rif.TB检测结果在52株分离株中的51株(98.1%)对RIF检测结果一致。Genotype MTBDR检测结果与52株分离株中的48株(92.3%)的测序结果相关,INNO-LiPA Rif.TB检测结果与52株分离株中的50株(96.2%)的测序结果相关。两种检测方法均有助于快速筛查疑似耐多药结核病患者的结核分枝杆菌分离株,但Genotype MTBDR检测方法具有能够同时检测对INH和RIF耐药性的优势。