小鼠和牛CXC趋化因子基因的定位

Assignment of the mouse and cow CXC chemokine genes.

作者信息

Modi W S, Amarante M R, Hanson M, Womack J E, Chidambaram A

机构信息

SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, MD, USA.

出版信息

Cytogenet Cell Genet. 1998;81(3-4):213-6. doi: 10.1159/000015033.

DOI:10.1159/000015033

PMID:9730606

Abstract

Gene specific PCR primers were constructed for five mouse and three bovine CXC chemokine genes. The mouse genes were assigned using SSCP analyses of the Jackson BSS backcross panel to two groups on chromosome 5. One group containing Gro1 and Mip2 cosegregated with reference markers Alb1 and Btc, and was positioned 2.2 cM proximal to a group comprising Ifi10, Mig, and Scyb5. The bovine genes IL8, GRO1, and GRO3, mapped using bovine x hamster somatic cell hybrids, were all found to be located on chromosome 6. The locations of these genes in these two animal species are consistent with the positions in humans (4q13-->q21), and previous syntenic relationships among these three mammals.

摘要

针对五个小鼠和三个牛的CXC趋化因子基因构建了基因特异性PCR引物。通过对杰克逊BSS回交群体进行SSCP分析，将小鼠基因定位到5号染色体上的两组。一组包含Gro1和Mip2，与参考标记Alb1和Btc共分离，并定位在包含Ifi10、Mig和Scyb5的一组近端2.2 cM处。使用牛×仓鼠体细胞杂种定位的牛基因IL8、GRO1和GRO3均位于6号染色体上。这些基因在这两种动物物种中的位置与人类（4q13→q21）中的位置以及这三种哺乳动物之间先前的同线性关系一致。