Neff M M, Chory J
Plant Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA.
Plant Physiol. 1998 Sep;118(1):27-35. doi: 10.1104/pp.118.1.27.
Single, double, and triple null combinations of Arabidopsis mutants lacking the photoreceptors phytochrome (phy) A (phyA-201), phyB (phyB-5), and cryptochrome (cry) 1 (hy4-2.23n) were examined for de-etiolation responses in high-fluence red, far-red, blue, and broad-spectrum white light. Cotyledon unhooking, unfolding, and expansion, hypocotyl growth, and the accumulation of chlorophylls and anthocyanin in 5-d-old seedlings were measured under each light condition and in the dark. phyA was the major photoreceptor/effector for most far-red-light responses, although phyB and cry1 modulated anthocyanin accumulation in a phyA-dependent manner. phyB was the major photoreceptor in red light, although cry1 acted as a phyA/phyB-dependent modulator of chlorophyll accumulation under these conditions. All three photoreceptors contributed to most blue light deetiolation responses, either redundantly or additively; however, phyB acted as a modulator of cotyledon expansion dependent on the presence of cry1. As reported previously, flowering time in long days was promoted by phyA and inhibited by phyB, with each suppressing the other's effect. In addition to the effector/modulator relationships described above, measurements of hypocotyls from blue-light-grown seedlings demonstrated phytochrome activity in blue light and cry1 activity in a phyAphyB mutant background.
研究了拟南芥缺乏光受体phytochrome（phy）A（phyA-201）、phyB（phyB-5）和cryptochrome（cry）1（hy4-2.23n）的单、双和三缺失突变体组合在高通量红光、远红光、蓝光和广谱白光下的去黄化反应。在每种光照条件下和黑暗中，测量了5日龄幼苗子叶的脱钩、展开和扩展、下胚轴生长以及叶绿素和花青素的积累。phyA是大多数远红光反应的主要光受体/效应器，尽管phyB和cry1以phyA依赖的方式调节花青素的积累。phyB是红光下的主要光受体，尽管在这些条件下cry1作为phyA/phyB依赖的叶绿素积累调节剂。所有三种光受体都以冗余或累加的方式参与了大多数蓝光去黄化反应；然而，phyB作为子叶扩展的调节剂，其作用依赖于cry1的存在。如先前报道，在长日照条件下，phyA促进开花时间，phyB抑制开花时间，二者相互抑制对方的作用。除了上述效应器/调节剂关系外，对蓝光培养的幼苗下胚轴的测量表明，蓝光中有phytochrome活性，在phyAphyB突变体背景中有cry1活性。
