Neff M M, Neff J D, Chory J, Pepper A E
Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Plant J. 1998 May;14(3):387-92. doi: 10.1046/j.1365-313x.1998.00124.x.
PCR-based detection of single nucleotide polymorphisms is a powerful tool for the plant geneticist. Cleaved amplified polymorphic sequence analysis is the most widely used approach for the detection of single nucleotide polymorphisms. However, this technique is limited to mutations which create or disrupt a restriction enzyme recognition site. This paper presents a modification of this technique where mismatches in a PCR primer are used to create a polymorphism based on the target mutation. This technique is useful for following known mutations in segregating populations and genetic mapping of isolated DNAs used for positional based cloning of new genes. In addition, a computer program has been developed that facilitates the design of these PCR primers.
基于聚合酶链反应（PCR）的单核苷酸多态性检测是植物遗传学家的一项强大工具。酶切扩增多态性序列分析是检测单核苷酸多态性最广泛使用的方法。然而，该技术仅限于能产生或破坏限制性内切酶识别位点的突变。本文介绍了对该技术的一种改进，即利用PCR引物中的错配来基于目标突变产生多态性。该技术对于追踪分离群体中的已知突变以及用于新基因定位克隆的分离DNA的遗传作图很有用。此外，还开发了一个计算机程序来辅助设计这些PCR引物。
