Grassly N C, Xiang Z, Ariyoshi K, Aaby P, Jensen H, Schim van der Loeff M, Dias F, Whittle H, Breuer J
Wellcome Trust Centre for the Epidemiology of Infectious Diseases, Department of Zoology, University of Oxford, Oxford, United Kingdom.
J Virol. 1998 Oct;72(10):7895-9. doi: 10.1128/JVI.72.10.7895-7899.1998.
We present the results of a 6-year study of 131 human immunodeficiency virus (HIV) type 2 (HIV-2)-infected individuals from a rural population in Guinea-Bissau. Proviral DNA sequences 1.3 kb in length were obtained from each individual and, together with clinical data, including proviral load and CD4 and CD8 levels, were used to assess whether viral genotype influences clinical outcome. With a phylogenetic model, a correlation was found between viral genotype and mortality; this correlation was not due to confounding factors, such as age-specific viral strains or cohabitation of patients. The data provide strong evidence for the involvement of viral genetic factors in determining HIV disease progression in vivo. The pattern of association found suggests that virulence factors are multiple and scattered throughout the HIV-2 genome and can be rapidly gained or lost by the virus through a combination of mutation and recombination. These findings may lead to the identification of viral determinants of HIV disease progression.
我们展示了一项针对来自几内亚比绍农村地区的131名感染2型人类免疫缺陷病毒（HIV-2）个体的为期6年的研究结果。从每个个体中获取了长度为1.3 kb的前病毒DNA序列，并将其与包括前病毒载量以及CD4和CD8水平在内的临床数据一起用于评估病毒基因型是否会影响临床结果。通过系统发育模型，发现病毒基因型与死亡率之间存在相关性；这种相关性并非由诸如特定年龄的病毒株或患者同居等混杂因素导致。这些数据为病毒遗传因素参与体内HIV疾病进展的决定过程提供了有力证据。所发现的关联模式表明，毒力因子是多样的，散布于整个HIV-2基因组中，并且病毒可通过突变和重组的组合迅速获得或丧失这些因子。这些发现可能会促成对HIV疾病进展的病毒决定因素的识别。
