Prost A C, Ménégaux F, Langlois P, Vidal J M, Koulibaly M, Jost J L, Duron J J, Chigot J P, Vayre P, Aurengo A, Legrand J C, Rosselin G, Gespach C
Service Central de Medecine Nucleaire, Centre Hospitalier Le Mans, 72037 Le-Mans, France.
Int J Oncol. 1998 Oct;13(4):871-5. doi: 10.3892/ijo.13.4.871.
Transferrin receptor density was investigated in human colorectal surgical specimens. Crude membranes were prepared from 23 cancer tumors (adenocarcinoma or malignant villous tumor) and 3 non-cancer tumors (polyadenoma or villous tumor) and 26 adjacent control mucosa. Contrary to non-cancer tumors, Scatchard analysis of 125I-transferrin binding data evidenced higher maximal transferrin binding capacity and lower dissociation constant in cancer tissues (Bmax cancer 1.828+/-0.320 nmol/g, Kd 24.1+/-4.7 nM), as compared to paired control colonic mucosa (Bmax contol 0.851+/-0.182 nmol/g, Kd 30.7+/-7.3 nM), paired t-tests: Bmax p<0.001, Kd p<0.05). As the cancer/control Bmax ratio was 2.6+/-0.4,transferrin carrier constructs should be proposed for cancer imaging or therapy.
