Neuhofer W, Müller E, Burger-Kentischer A, Beck F X
Department of Physiology, University of Munich, Germany.
Kidney Int Suppl. 1998 Sep;67:S165-7. doi: 10.1046/j.1523-1755.1998.06735.x.
High concentrations of NaCl are known to perturb the cytoskeleton. In this study, expression and intracellular localization of actin, an important component of the cytoskeleton and of heat shock protein (HSP)27, which promotes the assembly of F-actin, were examined in Madin-Darby canine kidney (MDCK) cells grown chronically in hypertonic medium. HSP27 mRNA abundance was increased twofold compared with wild-type MDCK cells. Chronic hypertonic stress led to enrichment of HSP27 in the insoluble component of the cell lysate and colocalization with cortical F-actin. These results support the notion that HSP27 participates in the modulation of actin dynamics following hypertonic stress.
已知高浓度的氯化钠会扰乱细胞骨架。在本研究中，对长期生长于高渗培养基中的犬肾上皮细胞（MDCK）中细胞骨架的重要组成部分肌动蛋白以及促进F-肌动蛋白组装的热休克蛋白（HSP）27的表达和细胞内定位进行了检测。与野生型MDCK细胞相比，HSP27 mRNA丰度增加了两倍。慢性高渗应激导致HSP27在细胞裂解物的不溶性组分中富集，并与皮质F-肌动蛋白共定位。这些结果支持了HSP27参与高渗应激后肌动蛋白动力学调节的观点。
