Miyasaki K T, Voganatsi A, Huynh T, Marcus M, Underwood S
Section of Oral Biology and the Dental Research Institute, UCLA School of Dentistry, Los Angeles, CA 90095-1668, USA.
J Periodontol. 1998 Aug;69(8):879-83. doi: 10.1902/jop.1998.69.8.879.
The purpose of this study was to determine the levels of calprotectin and lactoferrin, 2 microbiostatic proteins, in the gingival crevicular fluid (GCF) of normal children. The children represented a population, primarily underprivileged, seeking care at a regional dental health care center. GCF was collected from Ramfjord teeth (or their deciduous equivalent). GCF volume was quantified by conductance. Calprotectin and lactoferrin levels were quantified by sandwich ELISA, and found to have a mean value of 70.8+/-94.2 microg/mL and 68.2+/-108.7 microg/mL, respectively. The levels of calprotectin and lactoferrin varied directly with one another and inversely with the amount of fluid obtained in a 20-second sampling period. The mean levels were at or above the minimum inhibitory concentration determined in vitro.
本研究的目的是测定正常儿童龈沟液（GCF）中两种抑菌蛋白——钙卫蛋白和乳铁蛋白的水平。这些儿童主要是贫困人群，在一家地区性牙科保健中心寻求治疗。从Ramfjord牙（或其乳牙对应牙）采集GCF。通过电导对GCF体积进行定量。采用夹心ELISA法对钙卫蛋白和乳铁蛋白水平进行定量，结果发现其平均值分别为70.8±94.2微克/毫升和68.2±108.7微克/毫升。钙卫蛋白和乳铁蛋白水平彼此呈正相关，与在20秒采样期内获得的液体量呈负相关。平均水平达到或高于体外测定的最低抑菌浓度。
