Greene S P, Soucy D M, Song W C, Barber A E, Hagedorn F N, Illner H P, Shires G T
Department of Emergency Medicine, Texas Tech University Health Sciences Center, El Paso, USA.
Surgery. 1998 Sep;124(3):568-74.
Attempts to modify traditional fluid resuscitation have been based on animal models that evaluate several variables including anesthesia. This study presents the effects of early saline resuscitation from severe uncontrolled hemorrhage unanesthetized rats.
Sixty-three female Sprague-Dawley rats were equally divided into three groups: group A, nonresuscitated; and groups B and C, resuscitated ;with isotonic saline (40 and 80 mL/kg, respectively). Hemodynamics, blood loss, survival time, and mortality were recorded for 360 minutes after the hemorrhage, which was initiated by 75% resection of the tail.
In group C, 80 mL/kg of saline significantly lowered mortality (24% vs 76% and 71% for groups A and B, respectively) with concomitant increases in mean survival time (241 +/- 103 min vs 146 +/- 108 and 175 +/- 92 min for groups A and B, respectively). There were no statistically significant differences in blood loss, hematocrit, or hemodynamic parameters among the groups.
Early and adequate isotonic saline resuscitation of unanesthetized rats improved outcome despite continuing hemorrhage. The significantly lower mortality rate and increased survival time were not a result of transiently improved arterial pressure and did not correlate with blood loss. No significant bleeding increases were noted in the resuscitated groups.
对传统液体复苏进行改良的尝试是基于评估包括麻醉在内的多个变量的动物模型。本研究呈现了对未麻醉的大鼠进行严重失控性出血后早期生理盐水复苏的效果。
63只雌性斯普拉格 - 道利大鼠被平均分为三组：A组，未复苏组；B组和C组，复苏组，分别给予等渗盐水（40和80 mL/kg）。通过切除75%的尾巴引发出血后，记录360分钟内的血流动力学、失血量、存活时间和死亡率。
在C组中，80 mL/kg的盐水显著降低了死亡率（分别为24%，而A组和B组为76%和71%），同时平均存活时间增加（分别为241±103分钟，而A组和B组为146±108和175±92分钟）。各组之间在失血量、血细胞比容或血流动力学参数方面无统计学显著差异。
尽管持续出血，但对未麻醉的大鼠进行早期且充分的等渗盐水复苏可改善预后。死亡率显著降低和存活时间增加并非动脉压短暂改善的结果，且与失血量无关。在复苏组中未观察到失血量显著增加。
