Walder R, Liprandi F
Arch Virol. 1976;51(4):307-17. doi: 10.1007/BF01317934.
Thermal inactivation of Venezuelan Equine Encephalomyelitis Virus (VEEV) was studied at temperatures from 26 degrees to 55 degrees C. Inactivation of infectivity took place by two thermodynamically different reactions, one of which predominated at temperatures below 44 degrees C and the other at higher temperatures. The presence of 1 or 2 M NaCl stabilized the VEE virus at low temperatures but enhanced the inactivation at high temperatures. This latter effect at temperatures higher than 50 degrees C, is associated with the occurrence of two-component survival curves. The different effects of hypertonic NaCl concentrations at the two ranges of temperature, are related to different mechanisms of inactivation operating at each range (protein denaturation and nucleic acid-RNA breakdown). Different kinetics of thermal inactivation at 55 degrees C were observed between virus strains with different virulence. However, no significant correlations was found between the virulence of the eleven VEE virus strains studied and their thermostability at 37 degres and 55 degrees C.
研究了委内瑞拉马脑炎病毒（VEEV）在26摄氏度至55摄氏度温度下的热灭活情况。感染性的灭活通过两种热力学不同的反应发生，其中一种在44摄氏度以下的温度占主导，另一种在较高温度下占主导。1M或2M NaCl的存在在低温下稳定了VEE病毒，但在高温下增强了灭活作用。在高于50摄氏度的温度下，后一种效应与双组分存活曲线的出现有关。高渗NaCl浓度在两个温度范围内的不同效应，与每个范围内不同的灭活机制（蛋白质变性和核酸-RNA分解）有关。在具有不同毒力的病毒株之间观察到55摄氏度下热灭活的不同动力学。然而，在所研究的11株VEE病毒株的毒力与其在37摄氏度和55摄氏度下的热稳定性之间未发现显著相关性。
