海绵Sycon sp.产生超氧自由基的情况

Superoxide radical production by sponges Sycon sp.

作者信息

Peskin A V, Labas Y A, Tikhonov A N

机构信息

Institute of Developmental Biology, Russian Academy of Sciences, Moscow.

出版信息

FEBS Lett. 1998 Aug 28;434(1-2):201-4. doi: 10.1016/s0014-5793(98)00981-8.

DOI:10.1016/s0014-5793(98)00981-8

PMID:9738478

Abstract

Using the catechol Tiron as an O2-. scavenger, we showed that sea sponges (Sycon sp.) produce superoxide radicals in sea water at a high rate without any stimuli added. The rate of O2-. outflow from sponges to their water surroundings reaches a value of 0.5 nmol/min per sponge at pH 6.5. The generation of O2-. was inhibited by Cu,Zn-superoxide dismutase, and restored by the addition of KCN. We also confirmed the abiotic production of O2-. in sea water, detected earlier with a different method by Petasne and Zika [Nature 325 (1987) 516-518].

摘要

使用儿茶酚钛铁试剂作为超氧阴离子清除剂，我们发现海洋海绵（Sycon属）在未添加任何刺激物的情况下，能在海水中高速产生超氧自由基。在pH 6.5时，海绵向其周围水环境释放超氧阴离子的速率达到每块海绵0.5纳摩尔/分钟。超氧阴离子的产生受到铜锌超氧化物歧化酶的抑制，并通过添加氰化钾得以恢复。我们还证实了海水中超氧阴离子的非生物产生，这一现象此前由佩塔斯内和齐卡用不同方法检测到[《自然》325 (1987) 516 - 518]。