Davey K G, Johnson E M, Holmes A D, Szekely A, Warnock D W
PHLS Mycology Reference Laboratory, Public Health Laboratory, Kingsdown, Bristol, UK.
J Antimicrob Chemother. 1998 Aug;42(2):217-20. doi: 10.1093/jac/42.2.217.
The in-vitro susceptibilities of 143 isolates of Cryptococcus neoformans, collected from British patients between 1994 and 1996, to fluconazole and itraconazole were compared with those of 36 isolates collected between 1971 and 1985, 41 isolates collected between 1986 and 1989, and 43 Ugandan isolates collected in 1996. Testing was done with a broth microdilution method in YNB medium supplemented with glucose, incubation at 30 degrees C for 72 h, and an endpoint of 50% inhibition. The results showed that the MIC ranges, MIC50s and MIC90s of fluconazole and itraconazole for C. neoformans isolates from the UK have remained unchanged despite the recent widespread use of triazoles for long-term maintenance of patients with AIDS-associated cryptococcal meningitis. The MIC ranges, MIC50s and MIC90s of the 43 isolates from untreated Ugandan patients with AIDS were similar to those of the British isolates. Examination of our records for 1994-96 revealed six cases in which a four-fold or greater rise in the MIC of fluconazole was associated with relapsed cryptococcal meningitis.
对1994年至1996年间从英国患者中分离出的143株新型隐球菌的体外药敏情况进行了研究，比较了其对氟康唑和伊曲康唑的药敏情况，同时也对1971年至1985年间收集的36株、1986年至1989年间收集的41株以及1996年收集的43株乌干达分离株进行了同样的比较。采用肉汤微量稀释法在添加葡萄糖的YNB培养基中进行检测，在30℃下孵育72小时，以50%抑制率作为终点。结果显示，尽管最近三唑类药物被广泛用于艾滋病相关隐球菌性脑膜炎患者的长期维持治疗，但英国新型隐球菌分离株对氟康唑和伊曲康唑的MIC范围、MIC50和MIC90并未改变。43株来自未经治疗的乌干达艾滋病患者的分离株的MIC范围、MIC50和MIC90与英国分离株相似。查阅我们1994 - 96年的记录发现，有6例氟康唑MIC升高四倍或更多与隐球菌性脑膜炎复发有关。
