Ranjith-Kumar C T, Gopinath K, Jacob A N, Srividhya V, Elango P, Savithri H S
Department of Biochemistry, Indian Institute of Science, Banglore, India.
Arch Virol. 1998;143(8):1489-500. doi: 10.1007/s007050050392.
The genome of physalis mottle tymovirus (PhMV) is 6673 nucleotides long and is rich in cytosine residues (40.58%) like other tymoviruses. The organization of the genes is also similar to that of five other tymoviruses whose sequences are known. However, PhMV has the longest 3' noncoding region as well as the longest replicase (RP) ORF. The RP sequences are similar to those of other tymoviruses (48-60% identity) whereas the coat proteins (CP) and the overlapping proteins (OP) are conserved to a lesser extent (30-50% and 26-34% respectively). A tetra peptide "GILG" was found to be present in all the tymoviral OPs. The PhMV RP also possesses the methyl transferase, polymerase and the helicase motifs found in all the Sindbis-like super group of plant viruses. A phylogenetic analysis of the six tymoviral sequences revealed that they do not have a rigid hierarchical similarity relationship.
酸浆斑驳番茄病毒（PhMV）的基因组长度为6673个核苷酸，与其他番茄病毒一样富含胞嘧啶残基（40.58%）。其基因组织也与其他已知序列的五种番茄病毒相似。然而，PhMV具有最长的3'非编码区以及最长的复制酶（RP）开放阅读框。RP序列与其他番茄病毒的序列相似（同一性为48 - 60%），而外壳蛋白（CP）和重叠蛋白（OP）的保守程度较低（分别为30 - 50%和26 - 34%）。发现所有番茄病毒的OP中都存在一个四肽“GILG”。PhMV的RP还具有在所有类辛德毕斯植物病毒超群中都存在的甲基转移酶、聚合酶和解旋酶基序。对六个番茄病毒序列的系统发育分析表明，它们没有严格的层次相似关系。
