Ding S W, Keese P, Gibbs A
Research School of Biological Sciences, Australian National University, Canberra.
Virology. 1989 Oct;172(2):555-63. doi: 10.1016/0042-6822(89)90198-0.
The nucleotide sequence of the genome of ononis yellow mosaic tymovirus (OYMV) has been determined. The genome is single-stranded RNA, 6211 nucleotides long, and has three main open reading frames (ORFs), two of them overlapping. The largest ORF (nucleotides 179-5509) encodes a polyprotein of 1776 amino acid residues that has sequence similarities with polymerases of other viruses with RNA genomes. The smaller overlapping ORF (nucleotides 172-1965) encodes a protein of 597 amino acids of unknown function. The third ORF located at the 3' end of the genome (nucleotides 5487-6065) is the virion protein gene, and it overlaps by 20 nucleotides the 3' terminus of the largest ORF. The organization of the OYMV genome, its sequence, and the sequences of the protein it encodes are clearly similar to those of two other tymoviruses, turnip yellow mosaic virus and eggplant mosaic virus. The 5' terminal noncoding region of the OYMV genome is much longer than the same region of other tymoviral genomes and includes a direct duplication of a sequence of 21-23 nucleotides.
芒柄花黄花叶芜菁黄花叶病毒（OYMV）基因组的核苷酸序列已被测定。该基因组为单链RNA，长度为6211个核苷酸，有三个主要的开放阅读框（ORF），其中两个相互重叠。最大的开放阅读框（核苷酸179 - 5509）编码一个由1776个氨基酸残基组成的多蛋白，该多蛋白与其他具有RNA基因组的病毒的聚合酶具有序列相似性。较小的重叠开放阅读框（核苷酸172 - 1965）编码一个功能未知的由597个氨基酸组成的蛋白质。位于基因组3'端的第三个开放阅读框（核苷酸5487 - 6065）是病毒粒子蛋白基因，它与最大开放阅读框的3'末端重叠20个核苷酸。OYMV基因组的结构、其序列以及它所编码蛋白质的序列与另外两种芜菁黄花叶病毒，即芜菁黄花叶病毒和茄子花叶病毒的基因组、序列及编码蛋白质的序列明显相似。OYMV基因组的5'末端非编码区比其他芜菁黄花叶病毒基因组的同一区域长得多，并且包含一段21 - 23个核苷酸序列的直接重复。
