Jacob A N, Murthy M R, Savithri H S
Department of Biochemistry, Indian Institute of Science, Bangalore.
Arch Virol. 1992;123(3-4):367-77. doi: 10.1007/BF01317270.
The 3' terminal 1255 nt sequence of Physalis mottle virus (PhMV) genomic RNA has been determined from a set of overlapping cDNA clones. The open reading frame (ORF) at the 3' terminus corresponds to the amino acid sequence of the coat protein (CP) determined earlier except for the absence of the dipeptide, Lys-Leu, at position 110-111. In addition, the sequence upstream of the CP gene contains the message coding for 178 amino acid residues of the C-terminus of the putative replicase protein (RP). The sequence downstream of the CP gene contains an untranslated region whose terminal 80 nucleotides can be folded into a characteristic tRNA-like structure. A phylogenetic tree constructed after aligning separately the sequence of the CP, the replicase protein (RP) and the tRNA-like structure determined in this study with the corresponding sequences of other tymoviruses shows that PhMV wrongly named belladonna mottle virus [BDMV(I)] is a separate tymovirus and not another strain of BDMV(E) as originally envisaged. The phylogenetic tree in all the three cases is identical showing that any subset of genomic sequence of sufficient length can be used for establishing evolutionary relationships among tymoviruses.
通过一组重叠的 cDNA 克隆，已确定酸浆斑驳病毒（PhMV）基因组 RNA 的 3' 末端 1255 个核苷酸序列。3' 末端的开放阅读框（ORF）与先前确定的外壳蛋白（CP）的氨基酸序列相对应，只是在第 110 - 111 位缺少二肽 Lys - Leu。此外，CP 基因上游的序列包含编码假定复制酶蛋白（RP）C 末端 178 个氨基酸残基的信息。CP 基因下游的序列包含一个非翻译区，其末端 80 个核苷酸可折叠成特征性的类似 tRNA 的结构。将本研究中确定的 CP 序列、复制酶蛋白（RP）序列和类似 tRNA 结构分别与其他番茄病毒的相应序列进行比对后构建的系统发育树表明，错误命名为颠茄斑驳病毒[BDMV(I)]的 PhMV 是一种独立的番茄病毒，而非最初设想的 BDMV(E)的另一个毒株。在所有三种情况下的系统发育树都是相同的，这表明足够长度的基因组序列的任何子集都可用于建立番茄病毒之间的进化关系。
