Johne R, Müller H
Institut für Virologie, Veterinärmedizinische Fakultät, Universität Leipzig, Germany.
Arch Virol. 1998;143(8):1501-12. doi: 10.1007/s007050050393.
Avian polyomavirus (APV) infections have been reported to cause fatal disease in a wide range of psittacine species. Here we demonstrate APV infections in buzzards (Buteo buteo) and in a falcon (Falco tinnunculus) found dead in Germany, and in lovebirds (Agapornis pullaria) with fatal disease, wild-caught in Moçambique. APV infection in buzzards was determined by PCR amplification of parts of the viral genome followed by Southern blot hybridisation. The genomes of the isolates obtained from the falcon and one of the lovebirds proved to be very closely related to those of Budgerigar Fledgling Disease Virus (BFDV)-1, BFDV-2 and BFDV-3, isolated from budgerigar, chicken, and parakeet, respectively. A consensus sequence was delineated from the known nucleotide sequences of APV isolates. The significance of some nucleotide changes is discussed. Infectivity of all of these isolates was neutralized by antibodies directed against BFDV-1. Data presented in this investigation show that the polyomavirus isolates obtained from different avian species so far all belong to one genotype and one serotype within the proposed subgenus Avipolyomavirus of the family Papovaviridae. The designation Budgerigar Fledgling Disease Virus (BFDV) is, therefore, misleading as this virus type infects different species of birds. The name Avian Polymavirus and the abreviation APV should be adopted to all of the isolates investigated in detail at present. The possible role of birds of passage in the epidemiology in APV infections is discussed.
据报道，禽多瘤病毒（APV）感染可在多种鹦鹉科鸟类中引发致命疾病。在此，我们展示了在德国发现死亡的秃鹰（Buteo buteo）和猎鹰（Falco tinnunculus）以及在莫桑比克野生捕获的患有致命疾病的牡丹鹦鹉（Agapornis pullaria）中存在APV感染。通过对病毒基因组部分片段进行PCR扩增，随后进行Southern印迹杂交来确定秃鹰中的APV感染。从猎鹰和其中一只牡丹鹦鹉分离得到的病毒基因组被证明与分别从虎皮鹦鹉、鸡和长尾小鹦鹉中分离得到的虎皮鹦鹉雏鸟病病毒（BFDV）-1、BFDV-2和BFDV-3的基因组密切相关。从APV分离株的已知核苷酸序列中确定了一个共有序列。讨论了一些核苷酸变化的意义。所有这些分离株的感染性都被针对BFDV-1的抗体中和。本研究提供的数据表明，到目前为止从不同鸟类物种获得的多瘤病毒分离株都属于乳头多瘤空泡病毒科拟议的禽多瘤病毒亚属中的一个基因型和一个血清型。因此，虎皮鹦鹉雏鸟病病毒（BFDV）这一名称具有误导性，因为这种病毒类型可感染不同种类的鸟类。目前应将禽多瘤病毒这一名称及其缩写APV用于所有详细研究的分离株。文中还讨论了候鸟在APV感染流行病学中的可能作用。
