Bishop J W, Sims K L
Department of Pathology, Creighton University Medical Center, Saint Joseph Hospital, Omaha, Nebraska 68131, USA.
Anal Quant Cytol Histol. 1998 Aug;20(4):257-67.
To determine the feasibility and utility of thin-layer cytology preparations for morphometric analysis of nuclear and cytoplasmic area in cells from nongynecologic cytology specimens.
Identical paired samples from nongynecologic specimens (bronchial aspirate, urine, pleural and peritoneal fluid) were used to prepare thin-layer preparations and corresponding traditional membrane filter preparations. The paired preparations were analyzed by static image morphometry measuring eight nuclear and cytoplasmic parameters that allowed calculation of nuclear area, cytoplasmic area and total cell size. Hepatocytes and bronchial columnar, mesothelial, squamous and transitional cells were studied, as was a single case of high grade transitional cell carcinoma. Sufficient numbers of each cell type were measured to allow statistical analysis.
Both thin-layer and membrane filter preparation techniques yielded individual cells suitable for morphometric analysis, and there were no consistent morphologic measurement differences between the two methods. The thin-layer preparation had the following significant technical advantages: more numerous easily measured single cells, lack of interfering background and superior specimen stability. Benign oval to round nuclei from a variety of cell types have a mean nuclear area in a narrow range from 29 to 55 microns 2. The mean nuclear area of malignant cells studied was significantly larger (78 microns 2), and there was a significant decrease in absolute cytoplasmic profile area in the malignant population studied.
Thin-layer cytology preparations have significant advantages for morphometric studies over traditional membrane filter cytologic preparations. The morphometric measurement of nuclear area in benign and malignant cell populations has great potential as a generic screening tool for malignancy in cytologic specimens. Simultaneous measurement of cytoplasmic area adds a powerful dimension. The subsequent calculation of a true nuclear/cytoplasmic ratio may yield a sensitive and specific discriminator for detecting malignant cell populations in human nongynecologic cytology specimens.
确定薄层细胞学制片用于非妇科细胞学标本中细胞的细胞核和细胞质面积形态计量分析的可行性和实用性。
使用来自非妇科标本(支气管抽吸物、尿液、胸水和腹水)的相同配对样本制备薄层制片和相应的传统膜滤制片。通过静态图像形态计量学分析配对制片,测量八个细胞核和细胞质参数,从而计算细胞核面积、细胞质面积和细胞总体大小。研究了肝细胞、支气管柱状上皮细胞、间皮细胞、鳞状上皮细胞和移行细胞,以及一例高级别移行细胞癌。对每种细胞类型测量足够数量的细胞以进行统计分析。
薄层制片和膜滤制片技术均产生了适合形态计量分析的单个细胞,且两种方法之间在形态学测量上没有一致的差异。薄层制片具有以下显著的技术优势:易于测量的单个细胞数量更多、无干扰背景且标本稳定性更佳。来自多种细胞类型的良性椭圆形至圆形细胞核的平均核面积在29至55平方微米的狭窄范围内。所研究的恶性细胞的平均核面积显著更大(78平方微米),且在所研究的恶性细胞群体中,绝对细胞质轮廓面积显著减小。
与传统的膜滤细胞学制片相比,薄层细胞学制片在形态计量学研究中具有显著优势。良性和恶性细胞群体中细胞核面积的形态计量测量作为细胞学标本中恶性肿瘤的通用筛查工具具有巨大潜力。同时测量细胞质面积增加了一个有力的维度。随后计算真正的核/质比可能产生一种灵敏且特异的鉴别指标,用于检测人类非妇科细胞学标本中的恶性细胞群体。