Ito H, Hietala J, Blomqvist G, Halldin C, Farde L
Karolinska Institutet, Department of Clinical Neuroscience, Karolinska Hospital, Stockholm, Sweden.
J Cereb Blood Flow Metab. 1998 Sep;18(9):941-50. doi: 10.1097/00004647-199809000-00003.
Several approaches have been applied for quantification of D2 dopamine receptors in positron emission tomography studies using [11C]raclopride. Initial approaches were based on analyses of data obtained after rapid bolus injection of [11C]raclopride. A continuous infusion paradigm has more recently been applied. The current study compares these approaches in healthy men. Two positron emission tomography measurements were performed in each of six healthy men, the first with rapid bolus injection and the second with continuous infusion of [11C]raclopride. In rapid bolus injection, the binding potential was calculated by the following methods. One approach is the kinetic analysis using the standard three-compartment model. Another is to define a transient equilibrium at the moment when the specific binding reaches its maximum. In continuous infusion, binding potential was calculated by using time-activity data at equilibrium condition. All methods gave almost identical binding potential, representing cross-validation of these methods. The continuous infusion method can provide "true" equilibrium condition. The kinetic analysis is a sophisticated approach but requires determination of an arterial input function. The transient equilibrium method thus is suitable for routine clinical research, since it does not require determination of an arterial input function.
在使用[11C]雷氯必利的正电子发射断层扫描研究中，已应用多种方法来定量D2多巴胺受体。最初的方法基于对快速推注[11C]雷氯必利后获得的数据的分析。最近应用了连续输注模式。本研究在健康男性中比较了这些方法。对六名健康男性中的每一位进行了两次正电子发射断层扫描测量，第一次采用快速推注，第二次采用[11C]雷氯必利连续输注。在快速推注中，通过以下方法计算结合潜能。一种方法是使用标准三室模型进行动力学分析。另一种是在特异性结合达到最大值的时刻定义一个瞬态平衡。在连续输注中，通过使用平衡条件下的时间-活性数据来计算结合潜能。所有方法得出的结合潜能几乎相同，这代表了这些方法的交叉验证。连续输注方法可以提供“真正的”平衡条件。动力学分析是一种复杂的方法，但需要确定动脉输入函数。因此，瞬态平衡方法适用于常规临床研究，因为它不需要确定动脉输入函数。
