Angkasekwinai P, Looareesuwan S, Chaiyaroj S C
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1998 Mar;29(1):41-5.
The ability of Plasmodium falciparum infected erythrocytes from 162 Thai patients with uncomplicated malaria, 82 patients with severe malaria and 19 patients with cerebral malaria to form rosettes in vitro was studied. Of 263 isolates, 62 were evaluated for their adherence to different target molecules. We found that wide variation occurred in isolates from all groups in the level of rosette formation and adherence to CD36, intracellular adhesion molecule-1, thrombospondin and chondroitin sulfate A. No statistically significant correlation between the magnitude of rosette formation and disease severity was found (p > 0.05). In addition, our results from the use of purified CD36 as an adherence receptor showed no association between the degree rosetting and level of cytoadherence (p > 0.05, r = -0.04). Our data provide evidence that rosette formation and cytoadherence involve different molecular mechanisms and both phenomena can occur in all manifestations of the disease.
研究了来自162例非重症疟疾泰国患者、82例重症疟疾患者和19例脑型疟疾患者的恶性疟原虫感染红细胞在体外形成玫瑰花结的能力。在263个分离株中，对62个进行了其对不同靶分子黏附情况的评估。我们发现，所有组的分离株在玫瑰花结形成水平以及对CD36、细胞间黏附分子-1、血小板反应蛋白和硫酸软骨素A的黏附方面存在广泛差异。未发现玫瑰花结形成程度与疾病严重程度之间存在统计学显著相关性（p>0.05）。此外，我们使用纯化的CD36作为黏附受体的结果表明，玫瑰花结形成程度与细胞黏附水平之间无关联（p>0.05，r=-0.04）。我们的数据提供了证据，表明玫瑰花结形成和细胞黏附涉及不同的分子机制，且这两种现象可发生于该疾病的所有表现形式中。
