Kantama L, Jayanetra P, Pilantanapak A, Charaensub A
Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1998 Mar;29(1):85-90.
Salmonella serovar detection was studied by polymerase chain reaction (PCR). The primers were designed from Salmonella specific clone, A18:2 which was previously constructed and studied for genus specificity through colony hybridization. The primers were subsequently tested for specificity and sensitivity and showed that they amplified DNA fragment of all Salmonellae tested but did not amplify all isolates of non-Salmonellae tested. The amplified fragment was confirmed and increased sensitivity by nested PCR. Salmonella isolates amplified by the primers in the first round PCR were all positive in the second round. The sensitivity in the first and second round were 7 pg and 80 fg, respectively. The result indicated that the primers can be used as molecular tool for future field survey of Salmonella both in food and in clinical specimens.
采用聚合酶链反应（PCR）对沙门氏菌血清型进行检测。引物是根据沙门氏菌特异性克隆A18:2设计的，该克隆先前已构建，并通过菌落杂交研究了其属特异性。随后对引物的特异性和敏感性进行了测试，结果表明它们能扩增所有测试的沙门氏菌的DNA片段，但不能扩增所有测试的非沙门氏菌分离株。通过巢式PCR对扩增片段进行了确认并提高了敏感性。第一轮PCR中引物扩增出的沙门氏菌分离株在第二轮中均为阳性。第一轮和第二轮的敏感性分别为7 pg和80 fg。结果表明，这些引物可作为未来食品和临床标本中沙门氏菌现场调查的分子工具。
