弗氏白血病细胞红系分化过程中含Q-核糖核苷的转运RNA种类的特异性变化。

Specific changes in Q-ribonucleoside containing transfer RNA species during Friend leukemia cell erythroid differentiation.

作者信息

Lin V K, Farkas W R, Agris P F

出版信息

Nucleic Acids Res. 1980 Aug 11;8(15):3481-9. doi: 10.1093/nar/8.15.3481.

DOI:10.1093/nar/8.15.3481

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324165/

Abstract

Changes in specific tRNA isoacceptors during Friend leukemia cell (F.L.C.) erythroid differentiation have been found to be concomitant with differences in the extent of the Q-base modification in certain species of tRNA. Transfer RNA was isolated from F.L.C. cultures after 0, 36, 48, 72, and 96 hr of DMSO induced differentiation. Changes in 17 isoacceptors of tRNAasn, tRNAasp, tRNAhis and tRNAtyr were compared by RPC-5 chromatography. Isoacceptors of these tRNA changed in relative amounts, following consistent trends throughout cell differentiation. The amount and distribution of Q-base containing tRNA isoacceptors was assayed by measuring the quanine-tRNA transferase catalyzed incorporation of [3H]-labeled guanine into tRNA species undermodified in Q-base followed by RPC-5 chormatography of the tRNA. The amount of Q-base containing tRNA species decreased in the first 48 hr after the induction, then increased again, indicating the level of Q-modification is correlated to the process of differentiation. Isoacceptors that lacked the Q-base were eluted late from RPC-5.

摘要

已发现，在弗瑞德白血病细胞（F.L.C.）红系分化过程中，特定tRNA同工受体的变化与某些tRNA物种中Q碱基修饰程度的差异相伴发生。在二甲基亚砜诱导分化0、36、48、72和96小时后，从F.L.C.培养物中分离出转移RNA。通过RPC - 5色谱法比较了tRNAasn、tRNAasp、tRNAhis和tRNAtyr的17种同工受体的变化。这些tRNA的同工受体相对含量发生变化，在整个细胞分化过程中呈现出一致的趋势。通过测量鸟嘌呤 - tRNA转移酶催化将[3H]标记的鸟嘌呤掺入Q碱基修饰不足的tRNA物种中，然后对tRNA进行RPC - 5色谱分析，来测定含Q碱基的tRNA同工受体的数量和分布。诱导后的前48小时内，含Q碱基的tRNA物种数量减少，然后再次增加，这表明Q修饰水平与分化过程相关。缺乏Q碱基的同工受体从RPC - 5中洗脱较晚。

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本文引用的文献

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Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide.体外小鼠病毒诱导白血病细胞中的血红蛋白合成：二甲基亚砜对红系分化的刺激作用

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