Miteva V, Stefanova T, Budakov I, Ivanova I, Mitev V, Gancheva A, Ljubenov M
Institute of Microbiology, Bulg. Acad. Sciences, Sofia, Bulgaria.
Syst Appl Microbiol. 1998 Mar;21(1):151-61. doi: 10.1016/S0723-2020(98)80019-2.
A result of extensive screening of over 300 strains from the Collection of ELBY Bulgaricum, PLC, thirty six strains were selected as producers of bacteriocins, active closely related lactic acid bacterial species and some food spoilage bacteria. The selected strains belong to L. helveticus, L. bulgaricus and S. thermophilus, which are rare bacteriocin producers. Nineteen nonidentified producers were characterized by molecular taxonomic approaches--M13 fingerprinting, repetitive PCR, ribotyping and hybridization with species-specific probes, which allowed to affiliate them to the species L. delbrueckii. Several strains were found to harbour plasmids of different size. The estimated activity against food borne pathogens makes the isolated substances perspective as safe food preservatives and the producing strains could be used as components of starters with improved quality.
对保加利亚埃尔比公司保藏的300多株菌株进行广泛筛选的结果是，选择了36株作为细菌素的产生菌，这些细菌素对密切相关的乳酸菌和一些食品腐败细菌具有活性。所选菌株属于瑞士乳杆菌、保加利亚乳杆菌和嗜热链球菌，它们是罕见的细菌素产生菌。通过分子分类学方法——M13指纹图谱、重复PCR、核糖体分型以及与物种特异性探针杂交，对19株未鉴定的产生菌进行了鉴定，这使得它们可以归为德氏乳杆菌属。发现几株菌株含有不同大小的质粒。对食源性病原体的估计活性使得分离出的物质有望成为安全的食品防腐剂，并且产生菌株可以用作质量提高的发酵剂的成分。
