Zahner V, Momen H, Priest F G
Department of Biochemistry and Molecular Biology, Oswaldo Cruz Foundation, Manguinhos, Rio de Janeiro, Brazil.
Syst Appl Microbiol. 1998 Mar;21(1):162-70. doi: 10.1016/S0723-2020(98)80020-9.
Seventy six mosquito pathogenic strains of Bacillus sphaericus and 10 non-pathogens were examined by pulsed field gel electrophoresis (PFGE) of SmaI-digested chromosomal DNA. Non-pathogenic strains were clearly distinguished from the entomopathogenic types which were assigned to 21 groups (SmaI restriction patterns; SRPs). Some agreement between SRP based on PFGE and serotyping was noted, in particular all 39 strains of serotype 5a5b examined revealed identical SRPs indicating total conservation of the SmaI restriction site in these bacteria. Serotype 5a5b (SRP 12) strains comprise a widely distributed and abundant clonal lineage. Most serotypes, however, were divided into several SRPs. Seven strains from serotype 2a2b were covered in five SRPs in which toxin synthesis was correlated with chromosomal structure. Similarly, toxicity correlated with SRP in strains from serotypes 3 and 6.
采用脉冲场凝胶电泳（PFGE）技术对76株球形芽孢杆菌蚊虫致病菌株和10株非致病菌株的SmaI酶切染色体DNA进行分析。非致病菌株与昆虫致病型菌株明显区分，昆虫致病型菌株被分为21组（SmaI限制性酶切图谱；SRPs）。基于PFGE的SRP与血清分型之间存在一定一致性，特别是所有检测的39株血清型5a5b菌株显示出相同的SRP，表明这些细菌中SmaI酶切位点完全保守。血清型5a5b（SRP 12）菌株构成一个广泛分布且丰富的克隆谱系。然而，大多数血清型被分为几个SRP。血清型2a2b的7株菌株涵盖在5个SRP中，其中毒素合成与染色体结构相关。同样，血清型3和6的菌株中，毒性与SRP相关。
