利用多位点序列分型揭示球形芽孢杆菌的等位基因多样性和种群结构。
Allelic diversity and population structure of Bacillus sphaericus as revealed by multilocus sequence typing.
机构信息
Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
出版信息
Appl Environ Microbiol. 2011 Aug;77(15):5553-6. doi: 10.1128/AEM.00207-11. Epub 2011 Jun 17.
Abstract
The genetic diversity of 35 Bacillus sphaericus strains was analyzed by a newly developed multilocus sequence typing (MLST) scheme, toxin gene pool survey, and mosquito bioassay. The results demonstrated that strains assigned to the same sequence type (ST) had the same occurrence of toxin genes. Further sequence analysis revealed that toxic strains presented a nearly clonal population structure, whereas nontoxic strains had a high level of heterogeneity and were significantly distinct from toxic strains.
摘要
通过新开发的多位点序列分型（MLST）方案、毒素基因库调查和蚊虫生物测定，分析了 35 株球形芽孢杆菌的遗传多样性。结果表明，属于同一序列型（ST）的菌株具有相同的毒素基因发生。进一步的序列分析表明，有毒菌株呈现出近克隆的种群结构，而无毒菌株具有高度的异质性，与有毒菌株明显不同。
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