Gao Q, Berntzen G, Jiang R, Killie M K, Seljelid R
Department of Experimental Pathology, IMB, University of Tromsø, Norway.
Planta Med. 1998 Aug;64(6):551-4. doi: 10.1055/s-2006-957512.
A mannan (A) and a heteroglycan (D) were prepared by partial acidic hydrolysis of T3, a major polysaccharide fraction of the fungus Tremella fuciformis Berk. Methylation analysis of A and D showed that they contained a 1-->3 linked mannosyl main chain, to which were linked different side chains at position 2, 4, or 6 of the mannosyl residues. A and D were conjugated to albumin microbeads (AM) by reductive amination. The conjugates showed significant cytokine-stimulating activity in vitro whereas unconjugated AM had no activity. Not conjugated A and D showed cytokine-stimulating activity only in about 100 times higher concentrations.
通过对银耳（Tremella fuciformis Berk.）的主要多糖组分T3进行部分酸水解，制备了一种甘露聚糖（A）和一种杂聚糖（D）。对A和D的甲基化分析表明，它们含有一个1→3连接的甘露糖基主链，在甘露糖基残基的2、4或6位连接有不同的侧链。通过还原胺化反应将A和D与白蛋白微珠（AM）偶联。这些偶联物在体外显示出显著的细胞因子刺激活性，而未偶联的AM则无活性。未偶联的A和D仅在约高100倍的浓度下才显示出细胞因子刺激活性。
