Newton H
Centre for Reproduction, Growth and Development, Division of Obstetrics and Gynaecology, Leeds, UK.
Hum Reprod Update. 1998 May-Jun;4(3):237-47. doi: 10.1093/humupd/4.3.237.
The cryopreservation of ovarian tissue is a promising new method for conserving the fecundity of young cancer patients from the sterilizing effects of chemotherapy and/or radiotherapy. In murine and ovine studies the orthotopic insertion of frozen-thawed ovarian grafts into sterilized hosts has resulted in the birth of healthy offspring. Examination of human ovarian tissue after cryopreservation has shown that substantial numbers of morphologically normal and viable primordial follicles survive freeze-thawing. To date however, there is no efficient procedure for using the frozen-banked tissue to restore fertility to patients with ovarian failure. Autografting at an orthotopic or heterotopic site has the greatest potential for success but there is concern that the technique may reintroduce malignant cells to patients in remission from disease. Follicle isolation from the thawed tissue and growth to maturity in vitro is a preferable option but at present the technique is in its infancy. This review presents past and present research in the field of ovarian tissue cryopreservation and explores the possible strategies by which frozen-banked tissue from cancer patients could be used to restore fertility.
卵巢组织冷冻保存是一种很有前景的新方法，可用于保护年轻癌症患者的生育能力，使其免受化疗和/或放疗的绝育影响。在小鼠和绵羊研究中，将冻融后的卵巢移植物原位植入绝育宿主后，已成功诞下健康后代。对冷冻保存后的人类卵巢组织进行检查发现，大量形态正常且存活的原始卵泡在冻融后得以存活。然而，迄今为止，尚无有效的程序可利用冷冻保存的组织恢复卵巢功能衰竭患者的生育能力。在原位或异位进行自体移植成功的可能性最大，但人们担心该技术可能会将恶性细胞重新引入病情缓解的患者体内。从解冻后的组织中分离卵泡并在体外培养至成熟是一个更好的选择，但目前该技术尚处于起步阶段。本文综述了卵巢组织冷冻保存领域的过去和现在的研究，并探讨了利用癌症患者冷冻保存的组织恢复生育能力的可能策略。
