Bjørgaas M, Sagen E, Johnsen H, Vik T, Sager G
Department of Clinical Chemistry, University Hospital of Trondheim, Norway.
Scand J Clin Lab Invest. 1998 Jul;58(4):339-46. doi: 10.1080/00365519850186526.
Non-conjugated catecholamines were measured in morning urine samples from 111 healthy, non-hospitalized subjects aged 8-18 y and in 16 hospitalized, healthy subjects aged 12 16 y. The catecholamines were extracted by cation exchange columns and alumina adsorption and quantitated with HPLC with electrochemical detection. The concentration of catecholamines was related both to the urinary creatinine concentration and to the collecting period and body surface area. Linear regression analysis was used to estimate continuous age-related reference centiles based upon the measurements from the 111 non-hospitalized subjects. The upper limits for the adrenaline/creatinine and noradrenaline/creatinine ratios were lower than in previous studies. The excretion of adrenaline and noradrenaline per hour and m2 body surface area was higher in the 16 hospitalized than in the 74 age-matched non-hospitalized subjects. The excretion of the catecholamines expressed per hour and m2 body surface area and expressed relative to creatinine excretion, decreased with increasing age, and the excretion of adrenaline and noradrenaline per hour and m2 body surface area was higher in boys than in girls. In conclusion, standardization of urine sampling leads to more narrow ranges for urinary adrenaline and noradrenaline excretion in healthy children and adolescents.
对111名年龄在8至18岁的健康非住院受试者以及16名年龄在12至16岁的住院健康受试者的晨尿样本中的非共轭儿茶酚胺进行了测量。儿茶酚胺通过阳离子交换柱和氧化铝吸附进行提取，并用高效液相色谱-电化学检测法定量。儿茶酚胺的浓度与尿肌酐浓度、收集期和体表面积均相关。基于111名非住院受试者的测量结果，采用线性回归分析来估计与年龄相关的连续参考百分位数。肾上腺素/肌酐和去甲肾上腺素/肌酐比值的上限低于先前的研究。16名住院受试者每小时每平方米体表面积的肾上腺素和去甲肾上腺素排泄量高于74名年龄匹配的非住院受试者。每小时每平方米体表面积以及相对于肌酐排泄量而言的儿茶酚胺排泄量随年龄增长而降低，且男孩每小时每平方米体表面积的肾上腺素和去甲肾上腺素排泄量高于女孩。总之，尿液采样的标准化导致健康儿童和青少年尿肾上腺素和去甲肾上腺素排泄范围更窄。
