Trinkaus-Randall V, Nugent M A
Ophthalmology Department, Boston University, School of Medicine, MA 02118, USA.
J Control Release. 1998 Apr 30;53(1-3):205-14. doi: 10.1016/s0168-3659(97)00254-x.
We have designed a synthetic cornea that has a transparent hydrogel optic and a porous skirt. The device has been implanted in rabbit corneas. We have shown that keratocytes migrate into the device and deposit a complex extracellular matrix. The immediate response is detected in the surrounding stroma, and the secondary response is seen after cells have deposited a matrix in the disc. After implantation, a decrease in keratan sulfate accompanied by an increase in dermatan sulfate was detected in the surrounding tissue compared to the unwounded corneal stroma. The glycosaminoglycans in the disc resemble that of an injured stroma. The appearance of heparan sulfate and growth factors, bFGF and TGFbeta, was not detected until 6 weeks after implantation. The growth factors were detected at the interface between the device and the tissue and become more diffuse over time. Methods of controlled release in vivo were used to enhance the rate of fibroplasia and wound repair. While these were successful in the cornea itself, when combined with the synthetic cornea the response was magnified and the initial attempts yielded neovascularization and edema. Currently, efforts are being directed at controlling the release within the porous haptic so that fibroplasia is enhanced while minimizing an inflammatory response.
我们设计了一种合成角膜，它具有透明水凝胶光学部分和多孔裙边。该装置已植入兔角膜。我们已证明角膜细胞迁移到该装置中并沉积复杂的细胞外基质。在周围基质中检测到即时反应，在细胞在盘中沉积基质后观察到二次反应。植入后，与未受伤的角膜基质相比，周围组织中检测到硫酸角质素减少，同时硫酸皮肤素增加。盘中的糖胺聚糖类似于受伤基质的糖胺聚糖。直到植入后6周才检测到硫酸乙酰肝素以及生长因子bFGF和TGFβ的出现。生长因子在装置与组织的界面处被检测到，并随着时间的推移变得更加弥散。体内控释方法用于提高纤维增生和伤口修复的速度。虽然这些方法在角膜本身取得了成功，但与合成角膜结合时，反应会放大，最初的尝试导致了新生血管形成和水肿。目前，正在努力控制多孔触觉部分内的释放，以便在最小化炎症反应的同时增强纤维增生。
