Sato Y, Hirata T, Ogawa M, Fujisawa H
Division of Biological Science, Nagoya University Graduate School of Science, Chikusa-ku, Nagoya 464-8602, Japan.
J Neurosci. 1998 Oct 1;18(19):7800-10. doi: 10.1523/JNEUROSCI.18-19-07800.1998.
During development, mitral cells, the main output neurons of the olfactory bulb, project axons into a very narrow part of the telencephalon and form an axonal bundle called the lateral olfactory tract (LOT). The present study shows that before the first mitral cell axons elongate, the LOT position is already marked with a subset of early-generated neurons that are recognized by monoclonal antibody lot1 (lot cells). Mitral cell axons choose the lot cell position for their growth pathway and maintain a close contact with the cells until LOT formation is completed. Ablation of lot cells prevented LOT formation in organotypic culture. These results suggest that lot cells are "guidepost cells" for mitral cell axons.
在发育过程中，嗅球的主要输出神经元——二尖瓣细胞，将轴突投射到端脑的一个非常狭窄的区域，并形成一个名为外侧嗅束（LOT）的轴突束。本研究表明，在第一批二尖瓣细胞轴突伸长之前，LOT的位置就已经被一群早期生成的神经元所标记，这些神经元可被单克隆抗体lot1识别（lot细胞）。二尖瓣细胞轴突选择lot细胞的位置作为其生长路径，并与这些细胞保持密切接触，直到LOT形成完成。在器官型培养中，去除lot细胞可阻止LOT的形成。这些结果表明，lot细胞是二尖瓣细胞轴突的“路标细胞”。
