Dijkshoorn L, Van Harsselaar B, Tjernberg I, Bouvet P J, Vaneechoutte M
Department of Medical Microbiology, Leiden University Medical Center, The Netherlands.
Syst Appl Microbiol. 1998 Mar;21(1):33-9. doi: 10.1016/S0723-2020(98)80006-4.
Further to a previous study, the usefulness of amplified ribosomal DNA restriction analysis (ARDRA) for identification of Acinetobacter genomic species (DNA groups) was tested. A set of 202 Acinetobacter strains of 18 described genomic species and 17 unclassified strains were used. Restriction patterns obtained with a standard panel of restriction enzymes CfoI, AluI, MboI, RsaI and MspI allowed for separation of 11 DNA groups. With the additional use of restriction enzymes BfaI and BsmAI, five other (genomic) species could be differentiated, leaving only A. haemolyticus and DNA group 13BJ/14TU unseparated. With the standard panel of enzymes, ten new ARDRA profiles were noted in 14 unclassified strains. Two other unclassified strains had a profile in common with DNA group 15BJ, but were differentiated from this DNA group by restriction with bfaI. One remaining unclassified strain could not be differentiated from DNA group 17 by the standard panel of enzymes or by the enzymes BfaI and BsmAI. Results demonstrate the utility of ARDRA for identification of most genomic species of Acinetobacter. Furthermore, new ARDRA profiles that were shared by several unclassified strains may indicate so far undescribed genomic species in the genus.
在之前一项研究的基础上，对扩增核糖体DNA限制性分析（ARDRA）在不动杆菌基因组种（DNA群）鉴定中的实用性进行了测试。使用了一组包含18个已描述基因组种的202株不动杆菌菌株以及17株未分类菌株。用限制性内切酶CfoI、AluI、MboI、RsaI和MspI的标准组合获得的限制性图谱可区分出11个DNA群。额外使用限制性内切酶BfaI和BsmAI后，可区分出另外5个（基因组）种，仅溶血不动杆菌和DNA群13BJ/14TU无法区分。使用标准酶组合时，在14株未分类菌株中发现了10种新的ARDRA图谱。另外两株未分类菌株与DNA群15BJ具有相同的图谱，但通过BfaI酶切与该DNA群区分开来。剩下的一株未分类菌株，无论是使用标准酶组合还是BfaI和BsmAI酶，都无法与DNA群17区分开来。结果证明了ARDRA在不动杆菌大多数基因组种鉴定中的实用性。此外，几个未分类菌株共有的新ARDRA图谱可能表明该属中存在尚未描述的基因组种。
