囊性纤维化或慢性支气管炎患者痰液中铁和铁蛋白含量增加。

Increased iron and ferritin content of sputum from patients with cystic fibrosis or chronic bronchitis.

作者信息

Stites S W, Walters B, O'Brien-Ladner A R, Bailey K, Wesselius L J

机构信息

Division of Pulmonary and Critical Care Medicine, University of Kansas School of Medicine, Kansas City, USA.

出版信息

Chest. 1998 Sep;114(3):814-9. doi: 10.1378/chest.114.3.814.

DOI:10.1378/chest.114.3.814

PMID:9743172

Abstract

PURPOSE

Extracellular free iron, or iron bound to ferritin, may promote oxidative injury and bacterial growth in airways of patients with chronic airway inflammation due to cystic fibrosis (CF) or chronic bronchitis (CB). In this study, we assessed sputum content of total iron, ferritin, and transferrin in patients with CF or CB as well as sputum from normal subjects with acute airway inflammation caused by viral upper respiratory tract infections (URTIs).

METHODS

Spontaneously produced sputum was obtained from 33 subjects, including 10 subjects with CF, 18 subjects with CB (10 acute exacerbations, 8 with stable CB), and 5 subjects with URTIs (control subjects). After lysing and dilution, total iron concentrations were determined by controlled coulometry, ferritin was measured by radioimmunoassay, and transferrin was measured by enzyme-linked immunosorbent assay.

RESULTS

Iron was not present in detectable amounts in control sputums, but ferritin was present (6+/-2 ng/mg protein, mean+/-SE), as was transferrin (2.37+/-0.44 microg/mg). Compared with control subjects, concentrations of iron in sputum were increased in patient groups with higher amounts in CF patients (242+/-47 ng/mg, p<0.01) than CB patients with acute exacerbations or patients with stable CB (98+/-50 and 42+/-12 ng/mg, p<0.05 for both). Ferritin content of sputum was also increased in each group, with CF patients (113+/-22 ng/mg, p<0.001) higher than CB patients (acute, 45+/-10 ng/mg; stable, 87+/-24 ng/mg; p<0.01 for both). Compared with control subjects, sputum transferrin was decreased in CF patients (1.09+/-0.40 microg/mg, p<0.05), but not CB patients.

CONCLUSIONS

These findings indicate there are increased airway concentrations of total iron and ferritin-bound iron in patients with CB and, to a greater extent, in patients with CF. Particularly in CF patients who also demonstrated decreased airway concentrations of transferrin, ferritin-bound iron in airways may promote oxidative injury and enhance bacterial growth.

摘要

目的

细胞外游离铁或与铁蛋白结合的铁，可能会促进因囊性纤维化（CF）或慢性支气管炎（CB）导致慢性气道炎症患者气道中的氧化损伤和细菌生长。在本研究中，我们评估了CF或CB患者痰液中的总铁、铁蛋白和转铁蛋白含量，以及由病毒性上呼吸道感染（URTI）引起急性气道炎症的正常受试者的痰液情况。

方法

从33名受试者中获取自发咳出的痰液，其中包括10名CF患者、18名CB患者（10名急性加重期患者，8名稳定期CB患者）以及5名URTI患者（对照受试者）。在进行裂解和稀释后，通过控制库仑法测定总铁浓度，采用放射免疫分析法测定铁蛋白，并用酶联免疫吸附测定法测定转铁蛋白。

结果

对照痰液中未检测到铁，但存在铁蛋白（6±2 ng/mg蛋白质，平均值±标准误），转铁蛋白也存在（2.37±0.44 μg/mg）。与对照受试者相比，患者组痰液中的铁浓度升高，CF患者中的含量更高（242±47 ng/mg，p<0.01），高于急性加重期CB患者或稳定期CB患者（分别为98±50和42±12 ng/mg，两者p均<0.0 [疑似错误，原文为p<0.05 for both]）。每组痰液中的铁蛋白含量也升高，CF患者（113±22 ng/mg，p<0.001）高于CB患者（急性加重期，45±10 ng/mg；稳定期，87±24 ng/mg；两者p均<0.01）。与对照受试者相比，CF患者痰液中的转铁蛋白降低（1.09±0.40 μg/mg，p<0.05），但CB患者未降低。