Shimoda H, Matsuda H, Yamahara J, Yoshikawa M
Kyoto Pharmaceutical University, Japan.
Biol Pharm Bull. 1998 Aug;21(8):809-13. doi: 10.1248/bpb.21.809.
Immunomodulatory activities of constituents isolated from Hydrangeae Dulcis Folium and their related compounds on lymphocyte proliferation were investigated using splenocytes and lymph node cells. Thunberginol A (TA) significantly suppressed B lymphocyte proliferation stimulated by lipopolysaccharide (LPS) at 10(-5) M. However TA at a lower concentration (10(-6) M) and the other compounds, except hydrangenol and 3'-hydroxyhydrangeaic acid, tended to potentiate B lymphocytes proliferation (10(-5) M). On the other hand, thunberginol A significantly suppressed T lymphocyte proliferation stimulated by concanavalin A (Con A), but did not suppress phytohemagglutinin (PHA). Hydrocortisone and cyclosporin A strongly suppressed T lymphocyte proliferation induced by both Con A and PHA. These results suggest that thunberginol A acts on both B and T lymphocytes and may have a suppressive mechanism different from the known immunosuppressants. The cytotoxicity of TA for splenocytes seemed weaker than known immunosuppressants resulting from viability tests using MTT assay and the measurement of lactate dehydrogenase (LDH) released in the medium. Moreover, TA suppressed antigen-specific T lymphocyte proliferation in mice lymph node cells immunized with keyhole limpet hemocyanin (KLH). Thus, these findings show that TA has a suppressive effect on lymphocyte activation, and this inhibitory effect of TA seems to contribute to its suppressive effect on type IV allergies.
利用脾细胞和淋巴结细胞研究了甜茶（Hydrangeae Dulcis Folium）中分离出的成分及其相关化合物对淋巴细胞增殖的免疫调节活性。藤黄菌素A（TA）在10⁻⁵ M时能显著抑制脂多糖（LPS）刺激的B淋巴细胞增殖。然而，较低浓度（10⁻⁶ M）的TA以及除氢化藤黄酚和3'-羟基藤黄酸之外的其他化合物，在10⁻⁵ M时倾向于增强B淋巴细胞增殖。另一方面，藤黄菌素A显著抑制伴刀豆球蛋白A（Con A）刺激的T淋巴细胞增殖，但不抑制植物血凝素（PHA）刺激的T淋巴细胞增殖。氢化可的松和环孢素A强烈抑制Con A和PHA诱导的T淋巴细胞增殖。这些结果表明，藤黄菌素A对B淋巴细胞和T淋巴细胞均有作用，且可能具有与已知免疫抑制剂不同的抑制机制。通过MTT法进行活力测试以及测定培养基中释放的乳酸脱氢酶（LDH）发现，TA对脾细胞的细胞毒性似乎比已知免疫抑制剂弱。此外，TA抑制了用钥孔戚血蓝蛋白（KLH）免疫的小鼠淋巴结细胞中抗原特异性T淋巴细胞的增殖。因此，这些发现表明TA对淋巴细胞活化具有抑制作用，且TA的这种抑制作用似乎有助于其对IV型过敏的抑制作用。
