针对晚期糖基化终末产物结构的免疫化学方法：抗晚期糖基化终末产物抗体的特性分析

Immunochemical approaches to AGE-structures: characterization of anti-AGE antibodies.

作者信息

Ikeda K, Nagai R, Sakamoto T, Sano H, Araki T, Sakata N, Nakayama H, Yoshida M, Ueda S, Horiuchi S

机构信息

Department of Biochemistry, Kumamoto University, School of Medicine, Honjo, Japan.

出版信息

J Immunol Methods. 1998 Jun 1;215(1-2):95-104. doi: 10.1016/s0022-1759(98)00064-7.

DOI:10.1016/s0022-1759(98)00064-7

PMID:9744751

Abstract

Recent immunological approaches have greatly helped broaden our understanding of the biomedical significance of advanced glycation end products (AGEs) in aging and age-enhanced disease processes. Recently, Nepsilon-(carboxymethyl) lysine (CML), one of the glycoxidation products of AGEs, was demonstrated to be a major immunological epitope among AGEs. In the subsequent study, we characterized 13 different polyclonal anti-AGE antibodies and showed that these antibodies could be classified into three groups (Groups I, II and III). Group I was specific for CML and both Group II and Group III were specific for other epitopes (non-CML). Time-course study suggested that the epitope of Group II was formed earlier than that of Group III. In the present study, we prepared two monoclonal anti-AGE antibodies (2A2 and 3A3) whose epitope structures appeared to be closely related to Group III and Group II, respectively. The result indicates that AGE-proteins express at least two major non-CML epitopes.

摘要

最近的免疫学方法极大地帮助拓宽了我们对晚期糖基化终产物（AGEs）在衰老和年龄相关疾病进程中的生物医学意义的理解。最近，Nε-（羧甲基）赖氨酸（CML），AGEs的糖氧化产物之一，被证明是AGEs中的主要免疫表位。在随后的研究中，我们对13种不同的多克隆抗AGE抗体进行了表征，并表明这些抗体可分为三组（I组、II组和III组）。I组对CML具有特异性，II组和III组均对其他表位（非CML）具有特异性。时间进程研究表明，II组的表位比III组的表位形成得更早。在本研究中，我们制备了两种单克隆抗AGE抗体（2A2和3A3），其表位结构似乎分别与III组和II组密切相关。结果表明，AGE-蛋白质表达至少两种主要的非CML表位。