Komura Tomomi, Yamanaka Mikihiro, Nishimura Kohji, Hara Keita, Nishikawa Yoshikazu
Faculty of Human Life and Environment, Nara Women's University, Nara, Japan.
Graduate School of Human Life Science, Osaka City University, Osaka, Japan.
NPJ Aging Mech Dis. 2021 Jun 7;7(1):12. doi: 10.1038/s41514-021-00061-y.
To assess the utility of autofluorescence as a noninvasive biomarker of senescence in Caenorhabditis elegans, we measured the autofluorescence of individual nematodes using spectrofluorometry. The fluorescence of each worm increased with age. Animals with lower fluorescence intensity exhibited longer life expectancy. When proteins extracted from worms were incubated with sugars, the fluorescence intensity and the concentration of advanced glycation end products (AGEs) increased over time. Ribose enhanced these changes not only in vitro but also in vivo. The glycation blocker rifampicin suppressed this rise in fluorescence. High-resolution mass spectrometry revealed that vitellogenins accumulated in old worms, and glycated vitellogenins emitted six-fold higher fluorescence than naive vitellogenins. The increase in fluorescence with ageing originates from glycated substances, and therefore could serve as a useful noninvasive biomarker of AGEs. C. elegans can serve as a new model to look for anti-AGE factors and to study the relationship between AGEs and senescence.
为了评估自发荧光作为秀丽隐杆线虫衰老的非侵入性生物标志物的效用，我们使用荧光分光光度法测量了单个线虫的自发荧光。每条线虫的荧光随着年龄的增长而增加。荧光强度较低的动物预期寿命更长。当从线虫中提取的蛋白质与糖类一起孵育时，荧光强度和晚期糖基化终产物（AGEs）的浓度会随时间增加。核糖不仅在体外而且在体内都增强了这些变化。糖基化阻滞剂利福平抑制了荧光的这种升高。高分辨率质谱显示，卵黄蛋白原在老龄线虫中积累，糖基化的卵黄蛋白原发出的荧光比未糖基化的卵黄蛋白原高六倍。随着衰老而增加的荧光源自糖基化物质，因此可以作为AGEs有用的非侵入性生物标志物。秀丽隐杆线虫可以作为寻找抗AGEs因子以及研究AGEs与衰老之间关系的新模型。
