Sverdlov V E, Dukhanina O I, Hoebee B, Rapp J P
Department of Physiology and Molecular Medicine, Medical College of Ohio, 3035 Arlington Avenue, Toledo, Ohio 43614-5804, USA.
Mamm Genome. 1998 Oct;9(10):816-21. doi: 10.1007/s003359900873.
Fifty-eight new anonymous simple sequence repeats (SSR) were generated and mapped to various rat chromosomes. Among them two genes (rat homologs for human cadherin-14 and mouse fibroblast growth factor-related protein) were mapped on Chromosomes (Chrs) 2 and 11 respectively. The majority of markers were generated from a small insert genomic library specific to Chr 11, 13, 14, and 15. Twenty new markers were mapped to Chr 13, which is known to contain a blood pressure quantitative trait locus (QTL). Several approaches to obtain microsatellite markers are described. The protocols and newly generated markers should be useful for ongoing rat genome project.
生成了58个新的匿名简单序列重复序列（SSR），并将其定位到大鼠的不同染色体上。其中两个基因（人类钙黏蛋白-14和小鼠成纤维细胞生长因子相关蛋白的大鼠同源基因）分别定位在第2号和第11号染色体上。大多数标记是从特定于第11、13、14和15号染色体的小插入基因组文库中产生的。20个新标记被定位到第13号染色体上，已知该染色体包含一个血压数量性状位点（QTL）。描述了几种获得微卫星标记的方法。这些方案和新生成的标记对于正在进行的大鼠基因组计划应该是有用的。
