Shepel L A, Lan H, Brasic G M, Gheen M E, Hsu L C, Haag J D, Gould M N
Department of Human Oncology, University of Wisconsin-Madison, K4/334 CSC, 600 Highland Avenue, Madison, Wisconsin, USA.
Mamm Genome. 1998 Aug;9(8):622-8. doi: 10.1007/s003359900833.
Fifty-five novel rat microsatellite markers were isolated from libraries specific for rat chromosomes (Chrs) 1, 2, and 7. The markers were mapped in three backcross rat populations. Thirty of these markers mapped to Chrs 1, 2, or 7, while the other 25 mapped to other chromosomes. New markers for two genes, liver-specific transporter gene (Livtr) and insulin-responsive glucose transporter (Glut4), were also mapped to rat Chrs 9 and 10, respectively. Three provisionally assigned markers from previous studies were also confirmed. Detailed methodologies for the generation and enrichment of clones containing repeat sequences and for the isolation of chromosome-specific markers are presented, since they represent unique combinations and modifications of previous protocols. Such methods and the newly presented markers should be useful for both specific and general mapping studies in the rat.
从大鼠1号、2号和7号染色体特异性文库中分离出55个新型大鼠微卫星标记。这些标记在三个回交大鼠群体中进行了定位。其中30个标记定位于1号、2号或7号染色体，另外25个标记定位于其他染色体。两个基因（肝脏特异性转运蛋白基因（Livtr）和胰岛素反应性葡萄糖转运蛋白（Glut4））的新标记也分别定位于大鼠9号和10号染色体。先前研究中三个临时指定的标记也得到了确认。本文介绍了包含重复序列克隆的产生和富集以及染色体特异性标记分离的详细方法，因为它们代表了先前方案的独特组合和改进。这些方法和新提出的标记对于大鼠的特异性和一般性定位研究都应该是有用的。
