Du Y, Remmers E F, Zha H, Goldmuntz E A, Mathern P, Crofford L J, Szpirer J, Szpirer C, Wilder R L
Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892.
Cytogenet Cell Genet. 1995;68(1-2):107-11. doi: 10.1159/000133901.
Five genes and three anonymous DNA loci were mapped to rat chromosome 6 by genetic linkage and somatic cell hybrid analyses. The eight loci were all identified by PCR-based microsatellite polymorphism analysis and were characterized in 40 F2 intercross progeny of Fischer (F344/N) and Lewis (LEW/N) inbred rats for segregation analysis. These markers formed two linkage groups spanning, respectively, 58.1 cM and 4.0 cM. The first linkage group is comprised of two anonymous DNA loci and four genes with the following map order and distances: D6Cep8 (previously D3)-17.9 cM-D6Arb309-2.5 cM-Vsnl1 (neural visinin-like protein)-20.4 cM-Prkar2b (type IIb regulatory subunit of cAMP-dependent protein kinase)-8.8 cM-Fkhl1 (forkhead-like transcription factor BF-1)-8.5 cM-Rnu1c (18-3A U1 RNA). The second linkage group is comprised of one gene, Ckb (creatine kinase, brain) and one anonymous DNA locus, D6Arb54, separated by 4.0 cM. For each marker, two to eight alleles were detected in a panel of 16 inbred rat strains (ACI/N, BN/SsN, BUF/N, DA/Bk1, F344/N, LER/N, LEW/N, LOU/MN, MNR/N, MR/N, SHR/N, SR/Jr, SS/Jr, WBB1/N, WBB2/N, and WKY/N). Comparative mapping information indicated that rat chromosome 6 exhibits syntenic conservation with mouse chromosome 12. Homologs of the rat chromosome 6 loci have been identified on human chromosomes 2, 7, and 14.
通过遗传连锁和体细胞杂种分析，将五个基因和三个无名DNA位点定位到大鼠的6号染色体上。这八个位点均通过基于聚合酶链反应（PCR）的微卫星多态性分析鉴定，并在Fischer（F344/N）和Lewis（LEW/N）近交系大鼠的40个F2代杂交后代中进行特征分析以进行分离分析。这些标记形成了两个连锁群，分别跨越58.1厘摩（cM）和4.0厘摩。第一个连锁群由两个无名DNA位点和四个基因组成，其图谱顺序和距离如下：D6Cep8（以前的D3）-17.9厘摩-D6Arb309-2.5厘摩-Vsnl1（神经视宁蛋白样蛋白）-20.4厘摩-Prkar2b（cAMP依赖性蛋白激酶IIb型调节亚基）-8.8厘摩-Fkhl1（叉头样转录因子BF-1）-8.5厘摩-Rnu1c（18-3A U1 RNA）。第二个连锁群由一个基因Ckb（脑型肌酸激酶）和一个无名DNA位点D6Arb54组成，它们之间相隔4.0厘摩。对于每个标记，在一组16个近交大鼠品系（ACI/N、BN/SsN、BUF/N、DA/Bk1、F344/N、LER/N、LEW/N、LOU/MN、MNR/N、MR/N、SHR/N、SR/Jr、SS/Jr、WBB1/N、WBB2/N和WKY/N）中检测到两到八个等位基因。比较图谱信息表明，大鼠6号染色体与小鼠12号染色体表现出同线性保守性。在人类2号、7号和14号染色体上已鉴定出大鼠6号染色体位点的同源物。
