Hild M, Söderström T, Egberg N, Lundahl J
Department of Clinical Immunology, Karolinska Hospital, Stockholm, Sweden.
Vox Sang. 1998;75(1):18-25.
To measure bradykinin levels in platelet concentrates during and after leucocyte filtration.
Platelet concentrates were leukocyte depleted using three different leucocyte-depletion filters selected to represent filters with negative, positive or neutral charge, respectively. Bradykinin levels were analysed before, during and up to 90 min post-filtration.
Significant levels of bradykinin were generated when negatively charged leucocyte depletion filters were used. However, we found a high variation between platelet concentrates prepared from different donors as well as within the same concentrate when this was divided into three aliquots. Generated bradykinin was rapidly degraded in the collection bag and no bradykinin was detectable 60 min post-filtration.
Bradykinin generation is more pronounced when negatively charged leucocyte removal filters are used. Due to a rapid degradation of bradykinin in the storage bag, pre-storage filtration should minimise the risk of bradykinin related adverse reactions during transfusion with some filters.
检测白细胞过滤过程中及过滤后血小板浓缩物中的缓激肽水平。
使用三种不同的白细胞去除过滤器对血小板浓缩物进行白细胞去除，这三种过滤器分别代表带负电荷、正电荷或中性电荷的过滤器。在过滤前、过滤过程中及过滤后长达90分钟时分析缓激肽水平。
使用带负电荷的白细胞去除过滤器时会产生显著水平的缓激肽。然而，我们发现，由不同供体制备的血小板浓缩物之间以及同一浓缩物分成三份分装时，缓激肽水平存在很大差异。生成的缓激肽在收集袋中迅速降解，过滤后60分钟时检测不到缓激肽。
使用带负电荷的白细胞去除过滤器时，缓激肽生成更为明显。由于缓激肽在储存袋中迅速降解，对于某些过滤器，储存前过滤应能将输血过程中与缓激肽相关的不良反应风险降至最低。
