Neppert J, Bartz L, Clasen C
Institute of Transfusion Medicine, Clinics of the Christian Albrechts University, Kiel, Germany.
Vox Sang. 1998;75(1):70-1.
A patient experienced a severe haemolytic transfusion reaction. Neither the haemolytic property nor the specificity of the causative antibody had been sufficiently recognised when performing a microcolumn gel test.
Subsequent to the transfusion reaction, the serological property and specificity of the causative antibody were analysed. Tube and gel test methods were compared, as were various reagent red cell specimens and their constituents.
A haemolytic anti-Vel was detected in the tube test. In contrast, the particular commercial gel test kit used did not reveal the haemolytic property or specificity of the antibody. Our experiments suggest that this was apparently due to the presence of EDTA in the low ionic strength saline solution of the test kit.
In rare cases life-treatening haemolytic activity of an irregular blood group antibody may be undetected by a commericial microcolum gel test kit in which EDTA is a constituent.
一名患者发生了严重的溶血性输血反应。在进行微柱凝胶试验时，致病抗体的溶血特性和特异性均未得到充分识别。
输血反应发生后，对致病抗体的血清学特性和特异性进行了分析。比较了试管法和凝胶试验法，以及各种试剂红细胞标本及其成分。
在试管试验中检测到了抗-Vel溶血抗体。相比之下，所使用的特定商业凝胶试验试剂盒未显示该抗体的溶血特性或特异性。我们的实验表明，这显然是由于试剂盒的低离子强度盐溶液中存在乙二胺四乙酸（EDTA）。
在罕见情况下，含有EDTA成分的商业微柱凝胶试验试剂盒可能无法检测出不规则血型抗体的危及生命的溶血活性。
